Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4736714..4737211 Replicon chromosome
Accession NZ_CP076689
Organism Escherichia coli strain S21EC

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KQ260_RS23190 Protein ID WP_001173343.1
Coordinates 4737038..4737211 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag KQ260_RS23185 Protein ID WP_001312331.1
Coordinates 4736714..4737010 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ260_RS23175 (4732071) 4732071..4736000 + 3930 WP_001080129.1 autotransporter outer membrane beta-barrel domain-containing protein -
KQ260_RS23180 (4736214) 4736214..4736486 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
KQ260_RS23185 (4736714) 4736714..4737010 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KQ260_RS23190 (4737038) 4737038..4737211 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KQ260_RS23195 (4737330) 4737330..4738847 - 1518 WP_000003806.1 lysine--tRNA ligase -
KQ260_RS23200 (4739084) 4739084..4740541 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4721126..4746065 24939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T206259 WP_001173343.1 NZ_CP076689:4737038-4737211 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T206259 NZ_CP102247:c4724778-4724593 [Enterobacter asburiae]
GTGAAAAGTGCGGATGTCATTACTGTTCTGATCAGCCATGGCTGGAAATGTGTCAGAACGAAAGGAAGCCATCATCAGTT
TCGCCATCCCGAACAGAAAGGGCTGGTTACTGTCCCACATCCCAAGAAGGATATTAAACCGGGCACTCTCGCCCAGATCT
GGCGACAGGCGGGAATTAAACACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT206259 WP_001312331.1 NZ_CP076689:4736714-4737010 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT206259 NZ_CP102247:c4724578-4724171 [Enterobacter asburiae]
ATGTTTTATCCGGCTTACATTCATTCTGACTCCGACGGTAGCGCCAGCGGCTTTTTCCCTGACGTTCCAGGCTGTTTTTT
TGCAGGTGATTCCCTGGACGACGCTTTTCAGGATGCGCGTGATGCGCTCACAGCACACTTCGAAGCGCTATTTGAAATGG
ATAATGCACTGCCTCTTCCGGGTAATGTGGAAGCGCATCTGGAAAGTCGTCCAGAGGATTTCACAGGCGGGCAATGGCTG
TTGGTAGATATTAATATGAAGCAGTTCGACGGCAGGGCCGAGCGTATCAACATCACGATGCCTCGACGTTTGCTGGTAAA
AATTGATTCCTTTGTCAGCGAGCATCCTCAGTTCGGTAATCGAAGCGCATTTCTGGCCGAGGCAGCACGTCGCGTGCTGC
CTGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References