Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3922719..3923519 Replicon chromosome
Accession NZ_CP076689
Organism Escherichia coli strain S21EC

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag KQ260_RS19460 Protein ID WP_000342452.1
Coordinates 3922719..3923246 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag KQ260_RS19465 Protein ID WP_001277107.1
Coordinates 3923253..3923519 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ260_RS19435 (3917795) 3917795..3918562 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KQ260_RS19440 (3918559) 3918559..3919836 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
KQ260_RS19445 (3919833) 3919833..3920759 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KQ260_RS19450 (3920807) 3920807..3921916 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KQ260_RS19455 (3922339) 3922339..3922722 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
KQ260_RS19460 (3922719) 3922719..3923246 - 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KQ260_RS19465 (3923253) 3923253..3923519 - 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KQ260_RS19470 (3923668) 3923668..3924771 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KQ260_RS19475 (3925042) 3925042..3925896 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KQ260_RS19480 (3926141) 3926141..3927199 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
KQ260_RS19485 (3927192) 3927192..3927860 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T206257 WP_000342452.1 NZ_CP076689:c3923246-3922719 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T206257 NZ_CP102247:3796863-3797081 [Enterobacter asburiae]
ATGTCTGATAAACCACTCACAAAGATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTTGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCGGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAGCTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT206257 WP_001277107.1 NZ_CP076689:c3923519-3923253 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT206257 NZ_CP102247:3796461-3796835 [Enterobacter asburiae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAACCTTGATGAAAGCAACCATGGCTGGGTAAACGATCCAACGTCTGCTATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References