Detailed information of TA system TA206242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1747583..1749171
• Replicon: chromosome
• Accession: NZ_CP076689
• Organism: Escherichia coli strain S21EC

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A061YM43
• Locus tag: KQ260_RS08830
• Protein ID: WP_001125411.1
• Coordinates: 1747583..1748905 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A061YNW4
• Locus tag: KQ260_RS08835
• Protein ID: WP_001296076.1
• Coordinates: 1748905..1749171 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ260_RS08800 (1742604)	1742604..1744259	-	1656	WP_096944701.1	hypothetical protein	-
KQ260_RS08805 (1744264)	1744264..1744434	-	171	WP_000107046.1	hypothetical protein	-
KQ260_RS08810 (1744569)	1744569..1744817	-	249	WP_072129310.1	hypothetical protein	-
KQ260_RS08815 (1745231)	1745231..1745581	-	351	WP_216696421.1	single-stranded DNA-binding protein	-
KQ260_RS08820 (1745602)	1745602..1746726	-	1125	WP_216696424.1	phage/plasmid replication protein	-
KQ260_RS08825 (1746834)	1746834..1747160	+	327	WP_216696423.1	helix-turn-helix transcriptional regulator	-
KQ260_RS08830 (1747583)	1747583..1748905	-	1323	WP_001125411.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KQ260_RS08835 (1748905)	1748905..1749171	-	267	WP_001296076.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KQ260_RS08840 (1749479)	1749479..1750237	+	759	WP_001286537.1	trans-aconitate 2-methyltransferase	-
KQ260_RS08845 (1750241)	1750241..1751155	-	915	WP_216696425.1	bestrophin family protein	-
KQ260_RS08850 (1751352)	1751352..1752803	-	1452	WP_000854638.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49329.96 Da
• Isoelectric Point: 7.7906

>T206242 WP_001125411.1 NZ_CP076689:c1748905-1747583 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK

Nucleotide

• Length: 1323 bp

>T206242 NZ_CP102244:c4571220-4571047 [Escherichia coli O7:H15]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTTTACCAGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9987.34 Da
• Isoelectric Point: 4.9632

>AT206242 WP_001296076.1 NZ_CP076689:c1749171-1748905 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT206242 NZ_CP102244:c4571544-4571248 [Escherichia coli O7:H15]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGCATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A061YM43

Antitoxin

• AlphaFold DB: A0A061YNW4