Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 544840..545458 Replicon chromosome
Accession NZ_CP076689
Organism Escherichia coli strain S21EC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KQ260_RS02675 Protein ID WP_001291435.1
Coordinates 544840..545058 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KQ260_RS02680 Protein ID WP_000344800.1
Coordinates 545084..545458 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ260_RS02640 (540127) 540127..540699 + 573 WP_000779831.1 YbaY family lipoprotein -
KQ260_RS02645 (540730) 540730..541041 - 312 WP_000409908.1 MGMT family protein -
KQ260_RS02655 (541420) 541420..541773 + 354 WP_000878135.1 DUF1428 family protein -
KQ260_RS02660 (541815) 541815..543365 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KQ260_RS02665 (543529) 543529..543999 - 471 WP_000136192.1 YlaC family protein -
KQ260_RS02670 (544115) 544115..544666 - 552 WP_000102539.1 maltose O-acetyltransferase -
KQ260_RS02675 (544840) 544840..545058 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KQ260_RS02680 (545084) 545084..545458 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KQ260_RS02685 (546004) 546004..549153 - 3150 WP_001132478.1 efflux RND transporter permease AcrB -
KQ260_RS02690 (549176) 549176..550369 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T206236 WP_001291435.1 NZ_CP076689:c545058-544840 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T206236 NZ_CP102244:3749074-3749292 [Escherichia coli O7:H15]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT206236 WP_000344800.1 NZ_CP076689:c545458-545084 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT206236 NZ_CP102244:3748674-3749048 [Escherichia coli O7:H15]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References