Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 49181..49706 Replicon plasmid pS22EC_A
Accession NZ_CP076688
Organism Escherichia coli strain S22EC

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KQ258_RS24975 Protein ID WP_001159871.1
Coordinates 49401..49706 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag KQ258_RS24970 Protein ID WP_000813634.1
Coordinates 49181..49399 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ258_RS24965 (46218) 46218..46859 - 642 WP_000990667.1 hypothetical protein -
KQ258_RS24970 (49181) 49181..49399 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KQ258_RS24975 (49401) 49401..49706 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KQ258_RS24980 (49707) 49707..50516 + 810 WP_000016979.1 site-specific integrase -
KQ258_RS24985 (50654) 50654..50929 - 276 WP_000239529.1 hypothetical protein -
KQ258_RS24990 (50923) 50923..51567 - 645 WP_000633911.1 AAA family ATPase -
KQ258_RS24995 (51796) 51796..52767 + 972 WP_001103694.1 plasmid segregation protein ParM -
KQ258_RS25000 (52772) 52772..53164 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
KQ258_RS25005 (53169) 53169..54439 - 1271 Protein_56 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..116936 116936


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T206233 WP_001159871.1 NZ_CP076688:49401-49706 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T206233 NZ_CP102244:c2676322-2676146 [Escherichia coli O7:H15]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT206233 WP_000813634.1 NZ_CP076688:49181-49399 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT206233 NZ_CP102244:c2676100-2675684 [Escherichia coli O7:H15]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References