Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3903603..3904403 Replicon chromosome
Accession NZ_CP076687
Organism Escherichia coli strain S22EC

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag KQ258_RS19180 Protein ID WP_000342452.1
Coordinates 3903603..3904130 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag KQ258_RS19185 Protein ID WP_001277107.1
Coordinates 3904137..3904403 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ258_RS19155 (3898679) 3898679..3899446 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KQ258_RS19160 (3899443) 3899443..3900720 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
KQ258_RS19165 (3900717) 3900717..3901643 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KQ258_RS19170 (3901691) 3901691..3902800 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KQ258_RS19175 (3903223) 3903223..3903606 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
KQ258_RS19180 (3903603) 3903603..3904130 - 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KQ258_RS19185 (3904137) 3904137..3904403 - 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KQ258_RS19190 (3904552) 3904552..3905655 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KQ258_RS19195 (3905926) 3905926..3906780 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KQ258_RS19200 (3907025) 3907025..3908083 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
KQ258_RS19205 (3908076) 3908076..3908744 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T206228 WP_000342452.1 NZ_CP076687:c3904130-3903603 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T206228 NZ_CP102244:1127865-1128200 [Escherichia coli O7:H15]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT206228 WP_001277107.1 NZ_CP076687:c3904403-3904137 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT206228 NZ_CP102244:1127617-1127865 [Escherichia coli O7:H15]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References