Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3232007..3232661 Replicon chromosome
Accession NZ_CP076687
Organism Escherichia coli strain S22EC

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag KQ258_RS15825 Protein ID WP_000244781.1
Coordinates 3232007..3232414 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KQ258_RS15830 Protein ID WP_000354046.1
Coordinates 3232395..3232661 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ258_RS15805 (3227964) 3227964..3229697 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
KQ258_RS15810 (3229703) 3229703..3230413 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KQ258_RS15815 (3230438) 3230438..3231334 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KQ258_RS15820 (3231446) 3231446..3231967 + 522 WP_001055874.1 flavodoxin FldB -
KQ258_RS15825 (3232007) 3232007..3232414 - 408 WP_000244781.1 protein YgfX Toxin
KQ258_RS15830 (3232395) 3232395..3232661 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KQ258_RS15835 (3232904) 3232904..3233884 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
KQ258_RS15840 (3233961) 3233961..3234620 - 660 WP_000250274.1 hemolysin III family protein -
KQ258_RS15845 (3234784) 3234784..3235095 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
KQ258_RS15850 (3235140) 3235140..3236573 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T206224 WP_000244781.1 NZ_CP076687:c3232414-3232007 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T206224 NZ_CP102244:297088-297339 [Escherichia coli O7:H15]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT206224 WP_000354046.1 NZ_CP076687:c3232661-3232395 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT206224 NZ_CP102244:297360-297719 [Escherichia coli O7:H15]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCATACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References