Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2195474..2196305 Replicon chromosome
Accession NZ_CP076687
Organism Escherichia coli strain S22EC

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag KQ258_RS10975 Protein ID WP_000854815.1
Coordinates 2195931..2196305 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag KQ258_RS10970 Protein ID WP_001280918.1
Coordinates 2195474..2195842 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ258_RS10930 (2191065) 2191065..2191742 + 678 WP_001362823.1 hypothetical protein -
KQ258_RS10935 (2191758) 2191758..2192168 + 411 WP_000846703.1 hypothetical protein -
KQ258_RS10940 (2192389) 2192389..2193207 + 819 WP_001542275.1 DUF932 domain-containing protein -
KQ258_RS10945 (2193207) 2193207..2193452 + 246 WP_001164966.1 hypothetical protein -
KQ258_RS10950 (2193546) 2193546..2194019 + 474 WP_001542276.1 antirestriction protein -
KQ258_RS10955 (2194035) 2194035..2194511 + 477 WP_001186200.1 RadC family protein -
KQ258_RS10960 (2194574) 2194574..2194795 + 222 WP_000692345.1 DUF987 domain-containing protein -
KQ258_RS10965 (2194814) 2194814..2195458 + 645 WP_000086752.1 hypothetical protein -
KQ258_RS10970 (2195474) 2195474..2195842 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KQ258_RS10975 (2195931) 2195931..2196305 + 375 WP_000854815.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
KQ258_RS10980 (2196302) 2196302..2196496 + 195 WP_000988600.1 DUF5983 family protein -
KQ258_RS10985 (2196542) 2196542..2196622 + 81 Protein_2168 hypothetical protein -
KQ258_RS10990 (2196911) 2196911..2197057 - 147 Protein_2169 transposase domain-containing protein -
KQ258_RS10995 (2197159) 2197159..2197293 + 135 WP_059338447.1 EutP/PduV family microcompartment system protein -
KQ258_RS11000 (2197394) 2197394..2197723 - 330 WP_000450409.1 DUF496 family protein -
KQ258_RS11005 (2197895) 2197895..2198953 - 1059 WP_001200889.1 FUSC family protein -
KQ258_RS11010 (2199151) 2199151..2199624 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
KQ258_RS11015 (2199743) 2199743..2200909 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2173335..2245874 72539


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T206222 WP_000854815.1 NZ_CP076687:2195931-2196305 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T206222 NZ_CP102197:c1249504-1249259 [Schaalia odontolytica]
TTGTTTGCGAGGGAGCCTGTTCCTTCCCTTGATTCACGCATCATCCGCGTTGCGCTGCGCAAGTTGCGTCAGATCGATGC
GGCGTGTCATCTGGATGACCTCCGGATTCCTCCGGGCAACAGGCTCGAGCGTCTACGCGGTAATCGGGCGGGGCGGTACA
GTATCCGAATCAATGATCAGTGGCGTATCTGCTTCAGGTGGGGAGATGCTGGTCCAGAAGGGGTTGAGGTTGTTGACTAC
CACTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT206222 WP_001280918.1 NZ_CP076687:2195474-2195842 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT206222 NZ_CP102197:c1249227-1248964 [Schaalia odontolytica]
ATGGAAGACTTCATCAAGGGGTACGGAATTACGCAGCACAAGCTTGCCGTCGCGATCGGTGTTCCACCTCGCCGTATCAA
TGAAGTTGTCCACGGGAAGCGGGGAATTACGGCTGACACGGCGATGCGGCTGTCCCGTTACTTCGGTACGACCGCAGGAT
TCTGGATGAACCTGCAGATGCGCTATGAGCTCGATCGTGCTGAAGACACAATGGGTGAAACGCTGAGCGGAATCGTTCCA
CTTGCGGCGGTGGAAGTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References