Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 508013..508631 Replicon chromosome
Accession NZ_CP076687
Organism Escherichia coli strain S22EC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KQ258_RS02405 Protein ID WP_001291435.1
Coordinates 508013..508231 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KQ258_RS02410 Protein ID WP_000344800.1
Coordinates 508257..508631 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ258_RS02370 (503300) 503300..503872 + 573 WP_000779831.1 YbaY family lipoprotein -
KQ258_RS02375 (503903) 503903..504214 - 312 WP_000409908.1 MGMT family protein -
KQ258_RS02385 (504593) 504593..504946 + 354 WP_000878135.1 DUF1428 family protein -
KQ258_RS02390 (504988) 504988..506538 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KQ258_RS02395 (506702) 506702..507172 - 471 WP_000136192.1 YlaC family protein -
KQ258_RS02400 (507288) 507288..507839 - 552 WP_000102539.1 maltose O-acetyltransferase -
KQ258_RS02405 (508013) 508013..508231 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KQ258_RS02410 (508257) 508257..508631 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KQ258_RS02415 (509177) 509177..512326 - 3150 WP_001132478.1 efflux RND transporter permease AcrB -
KQ258_RS02420 (512349) 512349..513542 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T206207 WP_001291435.1 NZ_CP076687:c508231-508013 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T206207 NZ_CP102192:2010202-2010304 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT206207 WP_000344800.1 NZ_CP076687:c508631-508257 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT206207 NZ_CP102192:c2010311-2010166 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References