Detailed information of TA system TA206205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 55749..56269
• Replicon: chromosome
• Accession: NZ_CP076687
• Organism: Escherichia coli strain S22EC

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A061KX66
• Locus tag: KQ258_RS00255
• Protein ID: WP_001160964.1
• Coordinates: 55985..56269 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: KQ258_RS00250
• Protein ID: WP_000125566.1
• Coordinates: 55749..55982 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ258_RS00225 (50822)	50822..51109	+	288	WP_000203744.1	ferredoxin-like protein FixX	-
KQ258_RS00230 (51168)	51168..52499	+	1332	WP_001183209.1	MFS transporter	-
KQ258_RS00235 (52607)	52607..53137	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
KQ258_RS00240 (53130)	53130..54992	+	1863	WP_000377124.1	glutathione-regulated potassium-efflux system protein KefC	-
KQ258_RS00245 (55184)	55184..55663	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
KQ258_RS00250 (55749)	55749..55982	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
KQ258_RS00255 (55985)	55985..56269	+	285	WP_001160964.1	CcdB family protein	Toxin
KQ258_RS00260 (56296)	56296..57144	-	849	WP_000257181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
KQ258_RS00265 (57151)	57151..57528	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
KQ258_RS00270 (57531)	57531..58352	-	822	WP_001065365.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
KQ258_RS00275 (58349)	58349..59338	-	990	WP_000241227.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
KQ258_RS00280 (59338)	59338..60624	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-93)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10692.54 Da
• Isoelectric Point: 10.3030

>T206205 WP_001160964.1 NZ_CP076687:55985-56269 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Nucleotide

• Length: 285 bp

>T206205 NZ_CP102192:745313-745798 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT206205 WP_000125566.1 NZ_CP076687:55749-55982 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT206205 NZ_CP102192:745023-745316 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A061KX66

Antitoxin

• AlphaFold DB: A0A7U9LTM9