Detailed information of TA system TA206203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1146054..1147780
• Replicon: chromosome
• Accession: NZ_CP076683
• Organism: [Pseudomonas] zhaodongensis strain A252

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: KQ248_RS05430
• Protein ID: WP_216707044.1
• Coordinates: 1147130..1147780 (-)
• Length: 217 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: KQ248_RS05425
• Protein ID: WP_216707043.1
• Coordinates: 1146054..1147130 (-)
• Length: 359 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ248_RS23125	1141208..1141831	+	624	WP_253910916.1	hypothetical protein	-
KQ248_RS05415 (KQ248_05415)	1142077..1142784	-	708	WP_253910917.1	SprT family zinc-dependent metalloprotease	-
KQ248_RS05420 (KQ248_05420)	1142781..1146044	-	3264	WP_216707042.1	HsdR family type I site-specific deoxyribonuclease	-
KQ248_RS05425 (KQ248_05425)	1146054..1147130	-	1077	WP_216707043.1	macro domain-containing protein	Antitoxin
KQ248_RS05430 (KQ248_05430)	1147130..1147780	-	651	WP_216707044.1	DUF4433 domain-containing protein	Toxin
KQ248_RS05435 (KQ248_05435)	1147773..1149041	-	1269	WP_216707045.1	restriction endonuclease subunit S	-
KQ248_RS05440 (KQ248_05440)	1149034..1151805	-	2772	WP_216707046.1	class I SAM-dependent DNA methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (16-216)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 217 a.a.
• Molecular weight: 25048.68 Da
• Isoelectric Point: 7.9638

>T206203 WP_216707044.1 NZ_CP076683:c1147780-1147130 [[Pseudomonas] zhaodongensis]
MANHYPSLNPEKALIWRIIHRANLPWILDSGLHCGNSAVKAPDWVNIGNPELIDKRANHPVPLPPGGWLNDYVPFYFTPF
SPMLRNIHTGWGGIQKRPNEEIVILVSSLHHVGGLGLRFLFTDSHAYYQWANYYSDLADLNKIDWPLLQRRDFKRDPEDP
AKFERYQAEALIHRYLPVGGLLGIVCYTEALKQRIEQEIQARNLYLPVYARTGWYF

Nucleotide

• Length: 651 bp

>T206203 NZ_CP102192:329318-329686 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 359 a.a.
• Molecular weight: 40732.71 Da
• Isoelectric Point: 6.7664

>AT206203 WP_216707043.1 NZ_CP076683:c1147130-1146054 [[Pseudomonas] zhaodongensis]
MIRFTQGNLLEARAEALVNTVNTVGVMGKGIALMFKERFAENFRRYADACKAKEVQTGKMFVTQVHELDGPRWIVNFPTK
QHWRARSRMEWVVEGLQDLRRFLIEQQVKSVAIPPLGAGNGGLEWADVREQIERALGDLDIDILVFEPTKQYQNVAKRVG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIERYNPSDNPLDLKFVAHKYGPYANRLDHLLNNLDGSYLH
SEKRISDADPLDVIWFDDERRAFVQTYLKSEAKAYTQALESTAALIDGFESPFGMELLATVDWLLAREGVAPNVPALREG
LRHWRGGPDAAARKDRLFDDRALSIALERLTQPATALA

Nucleotide

• Length: 1077 bp

>AT206203 NZ_CP102192:329100-329321 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA