Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1803400..1803929 Replicon chromosome
Accession NZ_CP076660
Organism Staphylococcus aureus strain SG511 Berlin

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag KQU62_RS09020 Protein ID WP_000621176.1
Coordinates 1803400..1803762 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag KQU62_RS09025 Protein ID WP_000948331.1
Coordinates 1803759..1803929 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQU62_RS09000 (1800378) 1800378..1801148 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
KQU62_RS09005 (1801123) 1801123..1801602 - 480 WP_001190825.1 anti-sigma B factor RsbW -
KQU62_RS09010 (1801604) 1801604..1801930 - 327 WP_216701789.1 anti-sigma factor antagonist -
KQU62_RS09015 (1802049) 1802049..1803050 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
KQU62_RS09020 (1803400) 1803400..1803762 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KQU62_RS09025 (1803759) 1803759..1803929 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KQU62_RS09030 (1804014) 1804014..1805162 - 1149 WP_001281140.1 alanine racemase -
KQU62_RS09035 (1805228) 1805228..1805587 - 360 WP_000581197.1 holo-ACP synthase -
KQU62_RS09040 (1805591) 1805591..1806082 - 492 WP_001286801.1 PH domain-containing protein -
KQU62_RS09045 (1806075) 1806075..1807652 - 1578 WP_001294646.1 PH domain-containing protein -
KQU62_RS09050 (1807645) 1807645..1808124 - 480 WP_001287082.1 hypothetical protein -
KQU62_RS09055 (1808333) 1808333..1808893 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T206176 WP_000621176.1 NZ_CP076660:c1803762-1803400 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T206176 NZ_CP102179:4766120-4766422 [Pseudomonas canavaninivorans]
ATGAAGAAAATCGAATCCAGCAAATTCCGATCCTGGCTGAAAAAGCTCAAGGATGACACCGGCAGAGCTCGTATTACTTC
ACGGATCAATCGACTGATAGAGGGTTTACCTGGCGATGTCGCTCCAGTCGGCCATGGTCTCAGTGAGCTACGAATTCACT
ACGGGCCTGGCTACAGGGTGTACTTTTACCAGCAGGGCGATGTGCTGGTGATTCTACTCTGTGGCGGCGACAAGGGCAGT
CAGAGCCGCGACATTGAAACCGCCCACCAGATAATGCAGGCGTGGAGAGCACAAAATGACTGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT206176 WP_000948331.1 NZ_CP076660:c1803929-1803759 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT206176 NZ_CP102179:4766415-4766714 [Pseudomonas canavaninivorans]
ATGACTGAGACTTTTACAGAATTCGACCCCGCAGAATACCTCAGCACTCCCGAGGCCATCGCGGAGTTCATGAGCGATGC
CCTGGAAACCGGCGACGCAAGTTATATCGCCAAAGCGATGGGCGTAGTCGCACGAGCCAAGGGGATGACCGAATTGGCCA
GGGAAACGGGCCTGTCGCGCGAACAGCTATACCGTTCCTTCAGCGAGCACGGTAATCCCACTCTAAAAACACTTCTGGCG
GTCATGAAGGCACTTGGTGTCGATATGACGGCTCGGCCACACACGCACACTGCGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References