Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 72133..72668 Replicon plasmid unnamed
Accession NZ_CP076655
Organism Escherichia coli strain NC19

Toxin (Protein)


Gene name relE Uniprot ID V0VJJ7
Locus tag KPE96_RS23570 Protein ID WP_000222760.1
Coordinates 72381..72668 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag KPE96_RS23565 Protein ID WP_001132900.1
Coordinates 72133..72384 (+) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE96_RS23525 (67260) 67260..67820 + 561 WP_000139315.1 fertility inhibition protein FinO -
KPE96_RS23530 (67956) 67956..68168 + 213 WP_001299730.1 ANR family transcriptional regulator -
KPE96_RS23535 (68414) 68414..68488 + 75 Protein_70 endonuclease -
- (68744) 68744..68805 - 62 NuclAT_1 - -
- (68744) 68744..68805 - 62 NuclAT_1 - -
- (68744) 68744..68805 - 62 NuclAT_1 - -
- (68744) 68744..68805 - 62 NuclAT_1 - -
KPE96_RS23540 (68849) 68849..68998 + 150 WP_001312851.1 Hok/Gef family protein -
KPE96_RS23545 (69282) 69282..69539 + 258 WP_000083845.1 replication regulatory protein RepA -
KPE96_RS24235 (69556) 69556..69807 - 252 WP_223195197.1 replication protein RepA -
KPE96_RS24240 (69798) 69798..69845 + 48 WP_229471593.1 hypothetical protein -
KPE96_RS23555 (69838) 69838..70320 + 483 WP_001273588.1 hypothetical protein -
KPE96_RS23560 (70313) 70313..71170 + 858 WP_000774297.1 incFII family plasmid replication initiator RepA -
KPE96_RS23565 (72133) 72133..72384 + 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KPE96_RS23570 (72381) 72381..72668 + 288 WP_000222760.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KPE96_RS24245 (72772) 72772..73092 + 321 Protein_79 serine acetyltransferase -
KPE96_RS23575 (73108) 73108..73455 - 348 Protein_80 IS1-like element IS1A family transposase -
KPE96_RS23580 (73544) 73544..74566 + 1023 WP_000255956.1 IS21-like element IS100 family transposase -
KPE96_RS23585 (74563) 74563..75345 + 783 WP_001442137.1 IS21-like element IS100kyp family helper ATPase IstB -
KPE96_RS23590 (75400) 75400..75764 - 365 Protein_83 IS1 family transposase -
KPE96_RS23595 (75864) 75864..77085 + 1222 Protein_84 ISL3 family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sitABCD iutA / iucD / iucC / iucB / iucA / vat / iroN / iroE / iroD / iroC / iroB 1..166302 166302


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-83)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11105.15 Da        Isoelectric Point: 10.5832

>T206164 WP_000222760.1 NZ_CP076655:72381-72668 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T206164 NZ_CP102176:5378158-5378451 [Pseudomonas mediterranea]
ATGACCTATAGCCTCGAGTTTGATCGACGTGCATTAAAAGAATGGAACAAGCTAGGCGATACACTGCGACAACAGTTCAA
AAAGAAACTGATCGAGGTACTTGAAAACCCTCGCATCGAAGCCAATCGACTTAGACAACTTCCCGACTGCTACAAGTTGA
AATTGCGAAGCGCCGGGTATCGCCTGATCTACCAAGTTATTGATCAGGAAGTTGTGGTGTTCGTTGTTGCGATAGGAAAA
CGCGAGCGTGAAGCAGTTTATCAAGATGTGCAGGAGCGCATCTCATCCGATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT206164 WP_001132900.1 NZ_CP076655:72133-72384 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT206164 NZ_CP102176:5377929-5378168 [Pseudomonas mediterranea]
ATGGCTTCTATCAACATTCGCATCGACGACGACCTGAAGCAGCGATCATTCGCCGAGCTGGAGAAGCTCGGCGTCACACC
TTCCGAGTTGCTGCGTCAGACACTTCAGTACGTAGCAGAGCGAGGCAAGCTGCCTTTTAAAGCAGCTCTGCTCAGTGAAG
AAGACGAAGCACTCATTGCGGTGGTAACGGAGCGTCTTTCCAACCCTCAACGAGTGAAGGTCAGCCTGGATGACCTATAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0VJJ7


Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References