Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 38998..39523 Replicon plasmid unnamed
Accession NZ_CP076655
Organism Escherichia coli strain NC19

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KPE96_RS23375 Protein ID WP_001159871.1
Coordinates 39218..39523 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag KPE96_RS23370 Protein ID WP_000813630.1
Coordinates 38998..39216 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE96_RS23340 (34414) 34414..34830 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
KPE96_RS23345 (34827) 34827..35057 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
KPE96_RS23350 (35322) 35322..35822 + 501 WP_000528932.1 HEPN family nuclease -
KPE96_RS23355 (35835) 35835..36608 + 774 WP_000905949.1 hypothetical protein -
KPE96_RS23360 (36775) 36775..37908 + 1134 WP_000545986.1 DUF3800 domain-containing protein -
KPE96_RS23365 (37942) 37942..38454 - 513 WP_000151784.1 hypothetical protein -
KPE96_RS23370 (38998) 38998..39216 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KPE96_RS23375 (39218) 39218..39523 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KPE96_RS23380 (39524) 39524..40330 + 807 WP_000016968.1 site-specific integrase -
KPE96_RS23385 (41010) 41010..41741 - 732 WP_000504262.1 replication initiation protein -
KPE96_RS23390 (42362) 42362..43567 + 1206 WP_001442122.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sitABCD iutA / iucD / iucC / iucB / iucA / vat / iroN / iroE / iroD / iroC / iroB 1..166302 166302


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T206160 WP_001159871.1 NZ_CP076655:39218-39523 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T206160 NZ_CP102176:488096-488401 [Pseudomonas mediterranea]
ATGACGGAGATTATTCGAAGCTGTACCTTTTCCGGCTGGCTCTCAAAGCTGGCAGACAGCCGCGCCAGAATTCGCATTCA
GGTTCGAATCGATCGCATGGCTGATGGAAATCTGGGAGATGTCAAAGCTATAGGAGAAGGTTTGAGCGAAGCGCGTATCG
ATTATGGTCCCGGCTATCGAATCTATTTCATGCAGCAAGGCCGACAGTTGATCATTTTGCTGTGTGGCGGTGATAAAAGC
AGTCAGACCCGTGATATCAAACAAGCCAGGCTCATAGCGAAATCCTGGCAGGAGCAAAACCCATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT206160 WP_000813630.1 NZ_CP076655:38998-39216 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT206160 NZ_CP102176:488398-488685 [Pseudomonas mediterranea]
ATGACCGAACAATTCACTCGTTGGGACTCTGCCGAGTACCTCAAGACCGAAGAGGACATGGCCAATTATCTGGATGCATG
CATGGAGGAGGCCGGAGACGATCCCGCATTCATCGCAAAAGCCCTGGGGACCATTGCACGAGCTCGCGGAATGAGCCAAG
TGGCCCGTGATGCAGGGTTGTCACGTGAGAATCTTTATCGTGCCTTGTCGGGGGAAGGCAATCCTGAATTTGGAACCATT
TTGAAGGTTGTCAGAGCGTTGGGGTTGAAGCTGCATGCCAGTACTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References