Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 34414..35057 Replicon plasmid unnamed
Accession NZ_CP076655
Organism Escherichia coli strain NC19

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag KPE96_RS23340 Protein ID WP_001034046.1
Coordinates 34414..34830 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag KPE96_RS23345 Protein ID WP_001261278.1
Coordinates 34827..35057 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE96_RS23325 (29551) 29551..29967 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
KPE96_RS23330 (29964) 29964..30194 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KPE96_RS23335 (30575) 30575..34369 + 3795 WP_001144732.1 hypothetical protein -
KPE96_RS23340 (34414) 34414..34830 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
KPE96_RS23345 (34827) 34827..35057 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KPE96_RS23350 (35322) 35322..35822 + 501 WP_000528932.1 HEPN family nuclease -
KPE96_RS23355 (35835) 35835..36608 + 774 WP_000905949.1 hypothetical protein -
KPE96_RS23360 (36775) 36775..37908 + 1134 WP_000545986.1 DUF3800 domain-containing protein -
KPE96_RS23365 (37942) 37942..38454 - 513 WP_000151784.1 hypothetical protein -
KPE96_RS23370 (38998) 38998..39216 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
KPE96_RS23375 (39218) 39218..39523 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sitABCD iutA / iucD / iucC / iucB / iucA / vat / iroN / iroE / iroD / iroC / iroB 1..166302 166302


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T206159 WP_001034046.1 NZ_CP076655:c34830-34414 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T206159 NZ_CP102176:298698-298880 [Pseudomonas mediterranea]
ATGCGAAGTCGGGAAATGATCAGGAAGATCGAAGAAGACGGTTGGTATCTGGTGGCAGTGAAGGGTAGTCACCATCAGTA
CAAACATTCCGTCAAAACCGGAAGGGTGACAATCAAGCATCCTGATTCGGATCTGCCGAGGGGCACGATCAATAGCATAT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT206159 WP_001261278.1 NZ_CP076655:c35057-34827 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT206159 NZ_CP102176:298973-299374 [Pseudomonas mediterranea]
ATGAAATTTCCGGTCGTTCTGCACAAGGATGCCGATTCCGAATACGGAGTGACGGTTCCGGATGTTCCGGGCTGCTTCTC
CGCAGGCAGTACGGTGGCGCAGGCATTCGAAAACGTGAAGGAAGCGCTGTCGTTGCACTATGAGGGGTTGGTGGCAGACG
GCGAGCCACTGCCGCAAATCCATGAAGTCGACGACCATCTCGACAACCCCGAGTACGCCGGGGGGATTTGGGGTGTGGTT
GAGTTTGATGTGACGCCCTATTTTGGAAAGTCAGTGCGATTCAATGCGACGTTGCCCGAGCAGTTGCTTGAGCGCATCGA
CCAGACCGTAAGGAAGGATCAGCGCTACCGATCGCGCTCCGGCTTCCTGGCGGCGGCGGCCTTGCGCGAACTTTCGACCT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References