Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 29551..30194 Replicon plasmid unnamed
Accession NZ_CP076655
Organism Escherichia coli strain NC19

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KPE96_RS23325 Protein ID WP_001034044.1
Coordinates 29551..29967 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KPE96_RS23330 Protein ID WP_001261286.1
Coordinates 29964..30194 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE96_RS23305 (24684) 24684..25598 - 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -
KPE96_RS23310 (25954) 25954..26651 + 698 WP_223155668.1 IS1-like element IS1A family transposase -
KPE96_RS23315 (26905) 26905..27926 - 1022 Protein_25 DNA helicase UvrD -
KPE96_RS23320 (27911) 27911..29476 - 1566 WP_001128474.1 AAA family ATPase -
KPE96_RS23325 (29551) 29551..29967 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KPE96_RS23330 (29964) 29964..30194 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KPE96_RS23335 (30575) 30575..34369 + 3795 WP_001144732.1 hypothetical protein -
KPE96_RS23340 (34414) 34414..34830 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
KPE96_RS23345 (34827) 34827..35057 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sitABCD iutA / iucD / iucC / iucB / iucA / vat / iroN / iroE / iroD / iroC / iroB 1..166302 166302


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T206158 WP_001034044.1 NZ_CP076655:c29967-29551 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T206158 NZ_CP102175:c5627312-5626947 [Pseudomonas corrugata]
ATGAAATGGGATGTTGAATACACAGATGAGTTTGAGATTTGGTGGAACCGTCTCGGTGAGAGTGAGCAGGATTCTGTGCA
GGCCTCGGTAATGTTGCTGGGTGATGCCGGGCCTTTCTTGGGCTTTCCTCATACCAGCGATATTAAAGGTTCGCGTCATG
GCAACCTTCGGGAGCTACGGGTGCAGCATGGAGGAAGACCTTATCGTGTGCTCTATGCCTTCGACCCGCGTCGCTGCGCC
ATTCTTCTGATCGGCGGAGGCAAGACCGGCCAGGATCGCTGGTATCACGAGTACGTGCCTTTGGCCGAGCGTCTTTATGA
CGAGCATTTGGAAGTATTGAAGAAAGAGGGGTTTGACAATGGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT206158 WP_001261286.1 NZ_CP076655:c30194-29964 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT206158 NZ_CP102175:c5626954-5626643 [Pseudomonas corrugata]
ATGGCTAAGAAATTTGCCGATCTGGTGGCACGTCGTTCGCCAGATTCTCAGGTCCGCACGCAAGCGCTGTATCAGAAGTT
ACGGGCGGAAATGCCACTGCACGAGTTACGTCAGGCGCAGGAACTGAGCCAGGAAGCCTTGGCCAAGGCGCTGCATATCA
ACCAGGCGGCAGTCTCGAAAATGGAGCGTCGCACGGACATGTACATCAGCACGTTGCGTAACTACATCCGTGCCATGGGC
GGCGAATTGGAGATTATCGCCACGTTTCCGGATGGTCAGGTCAGGATCGAAAACTTCTCCCATCAACCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References