Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4333072..4333569 Replicon chromosome
Accession NZ_CP076654
Organism Escherichia coli strain NC19

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KPE96_RS20955 Protein ID WP_001173343.1
Coordinates 4333072..4333245 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag KPE96_RS20960 Protein ID WP_001425728.1
Coordinates 4333273..4333569 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE96_RS20945 (4329742) 4329742..4331199 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
KPE96_RS20950 (4331436) 4331436..4332953 + 1518 WP_001295074.1 lysine--tRNA ligase -
KPE96_RS20955 (4333072) 4333072..4333245 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KPE96_RS20960 (4333273) 4333273..4333569 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KPE96_RS20965 (4333796) 4333796..4334068 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KPE96_RS20970 (4334080) 4334080..4334310 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
KPE96_RS20975 (4334491) 4334491..4336122 + 1632 WP_001216487.1 sensor histidine kinase -
KPE96_RS20980 (4336119) 4336119..4336838 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T206157 WP_001173343.1 NZ_CP076654:c4333245-4333072 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T206157 NZ_CP102175:c4185152-4184865 [Pseudomonas corrugata]
ATGGCCGTTGAGTACGGAGTCGAGTGGGATCCGAAGGCGCTGAAAGAACTGCGAAAGCTCGACGGCGCCATCCGCCTCCA
GTTCTTGCGAAAGCTCCAAGAGCGGCAGGCCGCGCCACGAGTGCCAGGTGATGCACTTCACGGCATGAAGGATTGCTACA
AGATCAAATTACGCGGCGCTGGTTACCGGCTTGTATACCGTGTTGAGGATAAAAGGATTGTCATCCTGGTGCTGGCAGTA
GGCAAGCGGGAACGAAGCAGCGTTTACAATTCGGCCAAGAGTCGGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT206157 WP_001425728.1 NZ_CP076654:c4333569-4333273 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT206157 NZ_CP102175:c4185429-4185142 [Pseudomonas corrugata]
ATGCAAAACATTCTGGCCGATGTGGCCGTCAGCGTATCCGAGCTCAAGAAGAACCCCTCATCCGTCTTAAACGGTGCCAA
CGGCTTGCCCGTGGCCGTACTGAACCACAACCGAGTTATGGGCTACATGGTTCCGGCGAACGTCTACGAAGCGATGGTCG
AGCGCCTGGACGAATTGGAGCTCGTCCAACTGGTCAAGGCACGGCTCGACGCCAACGAAACACCTGTTCGAGTATCTCTC
AACGATCTCATTGGCGAGGCCGAGGCAGATATCGCCAATGGCCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References