Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4228494..4229089 Replicon chromosome
Accession NZ_CP076654
Organism Escherichia coli strain NC19

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag KPE96_RS20430 Protein ID WP_000239579.1
Coordinates 4228494..4228844 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag KPE96_RS20435 Protein ID WP_001223208.1
Coordinates 4228838..4229089 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE96_RS20410 (4223770) 4223770..4224792 - 1023 WP_001339490.1 ABC transporter permease -
KPE96_RS20415 (4224806) 4224806..4226308 - 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
KPE96_RS20420 (4226618) 4226618..4227574 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
KPE96_RS20425 (4227884) 4227884..4228414 + 531 WP_000055072.1 inorganic diphosphatase -
KPE96_RS20430 (4228494) 4228494..4228844 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
KPE96_RS20435 (4228838) 4228838..4229089 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
KPE96_RS20440 (4229302) 4229302..4229643 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
KPE96_RS20445 (4229646) 4229646..4233425 - 3780 WP_048943320.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T206156 WP_000239579.1 NZ_CP076654:c4228844-4228494 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T206156 NZ_CP102175:c331313-330897 [Pseudomonas corrugata]
ATGATCAAATTCATGCTGGATACCAACATCTGTATTTTCACCATCAAGAACAAACCCCAGATGGTCAGAGAAGCTTTCAA
TCTGCATCACGGCCAGCTTTGCATTAGTGCCGTTACGCTGATGGAGCTGATCTACGGCGCAGAAAAGTCTTGCGCCCCAG
AAAGAAATCTCGCCGTCATCGAAGGCTTCTGTGCTCGCCTTGAGGTTCTACCATTTGGTTATGAAGCTGCCGCACATACC
GGAATGATTCGAGCCGAACTGGCGAAGGCAGGCAAGCCAATCGGCCCTTACGACCAAATGATTGCCGGCCATGCCCGTTC
TCTCGGGCTGATCGTGATAACCAACAATCTGAAGGAGTTTGAAAGAGTTCCGGGACTGCGCACCGAAGATTGGACCGTTG
CGAAAGGGCCAGAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT206156 WP_001223208.1 NZ_CP076654:c4229089-4228838 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT206156 NZ_CP102175:c331544-331314 [Pseudomonas corrugata]
ATGGAACAAACCACCCTTTTCATGAGCAACCGCAGCCAAGCGGTCCGGCTTCCCAAAGCAGTGGCGATGCCAAGCGACGT
GAAGCGCGTAGATGTAATCGCCGTCGGCAGAGCCCGAATCATTACCCCGGCCGGAGAAGCATGGGACAGCTGGTTCGATG
GAGATACCGTGACAGCTGACTTTATGACAGACCGCGATCAGCCCGCCGATCAGGAGCGTGAACCCTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References