Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3537066..3537684 Replicon chromosome
Accession NZ_CP076654
Organism Escherichia coli strain NC19

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KPE96_RS17110 Protein ID WP_001291435.1
Coordinates 3537466..3537684 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KPE96_RS17105 Protein ID WP_000344800.1
Coordinates 3537066..3537440 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE96_RS17095 (3532155) 3532155..3533348 + 1194 WP_216696460.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KPE96_RS17100 (3533371) 3533371..3536520 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KPE96_RS17105 (3537066) 3537066..3537440 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KPE96_RS17110 (3537466) 3537466..3537684 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KPE96_RS17115 (3537856) 3537856..3538407 + 552 WP_000102568.1 maltose O-acetyltransferase -
KPE96_RS17120 (3538523) 3538523..3538993 + 471 WP_000136192.1 YlaC family protein -
KPE96_RS17125 (3539157) 3539157..3540707 + 1551 WP_048943370.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KPE96_RS17130 (3540749) 3540749..3541102 - 354 Protein_3352 DUF1428 family protein -
KPE96_RS17140 (3541481) 3541481..3541792 + 312 WP_000409911.1 MGMT family protein -
KPE96_RS17145 (3541823) 3541823..3542395 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T206155 WP_001291435.1 NZ_CP076654:3537466-3537684 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T206155 NZ_CP102174:c5849035-5848757 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT206155 WP_000344800.1 NZ_CP076654:3537066-3537440 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT206155 NZ_CP102174:c5848745-5848440 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References