Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 282840..283640 Replicon chromosome
Accession NZ_CP076654
Organism Escherichia coli strain NC19

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag KPE96_RS01310 Protein ID WP_000342449.1
Coordinates 283113..283640 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag KPE96_RS01305 Protein ID WP_001277108.1
Coordinates 282840..283106 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE96_RS01285 (278497) 278497..279165 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KPE96_RS01290 (279158) 279158..280216 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
KPE96_RS01295 (280461) 280461..281315 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KPE96_RS01300 (281587) 281587..282690 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KPE96_RS01305 (282840) 282840..283106 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KPE96_RS01310 (283113) 283113..283640 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KPE96_RS01315 (283637) 283637..284020 - 384 WP_000778777.1 aspartate 1-decarboxylase autocleavage activator PanM -
KPE96_RS01320 (284444) 284444..285553 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KPE96_RS01325 (285601) 285601..286527 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KPE96_RS01330 (286524) 286524..287801 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
KPE96_RS01335 (287798) 287798..288565 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T206137 WP_000342449.1 NZ_CP076654:283113-283640 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T206137 NZ_CP102149:1358867-1359127 [Streptococcus suis]
ATGTACCGTATTGACTATTCCAAAAGAGCACAGCGTCAGATTCGTAAAATGGATAAGCAGGTACAGAGGTTACTATTTAC
CTGGATTGACAAACATCTGGACGGTACAGAAAATCCTAGGCAGCATGGCAAGGGCTTGACAGATAACCGAGCTAATGAAT
GGGGCTATCGTATCGGCGATTACAGACTGATTTACGATATTCAAGAGGATAAGCTGGTTATTCTGGCCCTTGAGTTTGGA
CACCGTAAAGATATTTATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT206137 WP_001277108.1 NZ_CP076654:282840-283106 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT206137 NZ_CP102149:1358644-1358865 [Streptococcus suis]
ATGAGTACAGTGACAGTTCGATTGAACCAAGAAGAGGAAACTTTTTTCAAAAGTTACGCGCAGTTGACAGGCCAAAGCCT
CTCGAGTTTATTTAAGAAGGCGCTGGAGCGGACTATAGAAGATGAGTTTGATTTAAAAATCTACCACCAAGCCTATAAGG
AGTACCAAGACGACCCTGAAACCATTTCGCACGCTGACTTCAAGAAAGAGCTAGGTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References