Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2919970..2920612 Replicon chromosome
Accession NZ_CP076653
Organism Bacillus sp. NP247

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag KPL75_RS15450 Protein ID WP_000635965.1
Coordinates 2920262..2920612 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag KPL75_RS15445 Protein ID WP_000004570.1
Coordinates 2919970..2920257 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPL75_RS15420 (KPL75_15415) 2915175..2916137 + 963 WP_219916893.1 UV DNA damage repair endonuclease UvsE -
KPL75_RS15425 (KPL75_15420) 2916264..2916812 - 549 WP_219916894.1 rhomboid family intramembrane serine protease -
KPL75_RS15430 (KPL75_15425) 2916905..2917264 + 360 WP_002029438.1 holo-ACP synthase -
KPL75_RS15435 (KPL75_15430) 2917421..2918371 + 951 WP_219921113.1 outer membrane lipoprotein carrier protein LolA -
KPL75_RS15440 (KPL75_15435) 2918489..2919658 + 1170 WP_002151404.1 alanine racemase -
KPL75_RS15445 (KPL75_15440) 2919970..2920257 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
KPL75_RS15450 (KPL75_15445) 2920262..2920612 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KPL75_RS15455 (KPL75_15450) 2920681..2922849 + 2169 WP_219921114.1 Tex family protein -
KPL75_RS15460 (KPL75_15455) 2922908..2923024 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
KPL75_RS15465 (KPL75_15460) 2923234..2923689 + 456 WP_219916895.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T206134 WP_000635965.1 NZ_CP076653:2920262-2920612 [Bacillus sp. NP247]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T206134 NZ_CP102149:52838-53104 [Streptococcus suis]
ATGAAGACCTGTTATAAACTCGTACCGACCAGTCGTTTTATCAAACAGTTGAAAAAGTTGGACAAATTTACCCAAAAACA
GATTACCAACTACCTGTCTAGTCATGTGACCGACAACCCCAGACAATACGGAAAAGCCTTGACTGCTAATCGTTCTGGCC
AATGGCGCTATCGTATCGGCAACTACAGGGTTATCGTCAGTATTGAAGATGACAAATTGATTGTAACGGCCCTTGAAGTC
GGGCATCGGAAAGAAATATATAAATAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT206134 WP_000004570.1 NZ_CP076653:2919970-2920257 [Bacillus sp. NP247]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT206134 NZ_CP102149:52603-52854 [Streptococcus suis]
ATGGCAGCTATTACATTGAAAGTTTCTGAGCAGGACAAGCTCTTTATGCAGGCTATGGCAAAGTTTGAAGGTGTGAGTTT
GTCAGAACTCATTCGGACTAAAACGCTTGAAGCCCTTGAAGATGAATATGATGCCCGTGTGGCAGACATCGCTCTAGCTG
AATACGAGGCAGACTTGGCGAAAGGCATTGAACCGATTAGCTGGGAGGAAATGTTGGAAGAGTTGGGGTTGACGGATGAA
GACCTGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References