Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2148243..2148757 Replicon chromosome
Accession NC_002953
Organism Staphylococcus aureus subsp. aureus MSSA476

Toxin (Protein)


Gene name TsdT Uniprot ID A0A641A3V2
Locus tag SAS_RS10820 Protein ID WP_000581793.1
Coordinates 2148243..2148452 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag SAS_RS10825 Protein ID WP_000138932.1
Coordinates 2148464..2148757 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAS_RS10800 2143773..2145131 - 1359 WP_000611483.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
SAS_RS10805 2145146..2146216 - 1071 WP_000159627.1 D-alanine--D-alanine ligase -
SAS_RS10810 2146513..2147715 + 1203 WP_001109939.1 rod shape-determining protein RodA -
SAS_RS10815 2147978..2148115 - 138 WP_000828354.1 hypothetical protein -
SAS_RS10820 2148243..2148452 - 210 WP_000581793.1 heavy-metal-associated domain-containing protein Toxin
SAS_RS10825 2148464..2148757 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
SAS_RS10830 2148923..2150407 + 1485 WP_000571549.1 cardiolipin synthase -
SAS_RS10835 2150435..2151082 + 648 WP_001187628.1 HD domain-containing protein -
SAS_RS10845 2151696..2152568 - 873 WP_000725802.1 membrane protein insertase YidC -
SAS_RS10850 2152654..2153295 - 642 WP_000483155.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7809.75 Da        Isoelectric Point: 4.1008

>T20613 WP_000581793.1 NC_002953:c2148452-2148243 [Staphylococcus aureus subsp. aureus MSSA476]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVI

Download         Length: 210 bp

>T20613 NC_002953:c2148452-2148243 [Staphylococcus aureus subsp. aureus MSSA476]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTAATTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT20613 WP_000138932.1 NC_002953:c2148757-2148464 [Staphylococcus aureus subsp. aureus MSSA476]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT20613 NC_002953:c2148757-2148464 [Staphylococcus aureus subsp. aureus MSSA476]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCGATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A641A3V2

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References