Detailed information of TA system TA020612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2128508..2129037
• Replicon: chromosome
• Accession: NC_002953
• Organism: Staphylococcus aureus subsp. aureus MSSA476

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAS_RS10730
• Protein ID: WP_000621175.1
• Coordinates: 2128508..2128870 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAS_RS10735
• Protein ID: WP_000948331.1
• Coordinates: 2128867..2129037 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAS_RS10705	2125487..2126257	-	771	WP_001041108.1	RNA polymerase sigma factor SigB	-
SAS_RS10710	2126232..2126711	-	480	WP_001190830.1	anti-sigma B factor RsbW	-
SAS_RS10715	2126713..2127039	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAS_RS10720	2127158..2128159	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAS_RS10730	2128508..2128870	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAS_RS10735	2128867..2129037	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAS_RS10740	2129122..2130270	-	1149	WP_001281154.1	alanine racemase	-
SAS_RS10745	2130336..2130695	-	360	WP_000581197.1	holo-ACP synthase	-
SAS_RS10750	2130699..2131190	-	492	WP_001205912.1	PH domain-containing protein	-
SAS_RS10755	2131177..2132760	-	1584	WP_001294626.1	PH domain-containing protein	-
SAS_RS10760	2132753..2133232	-	480	WP_001287078.1	hypothetical protein	-
SAS_RS10765	2133441..2134001	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T20612 WP_000621175.1 NC_002953:c2128870-2128508 [Staphylococcus aureus subsp. aureus MSSA476]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T20612 NC_002953:c2128870-2128508 [Staphylococcus aureus subsp. aureus MSSA476]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT20612 WP_000948331.1 NC_002953:c2129037-2128867 [Staphylococcus aureus subsp. aureus MSSA476]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT20612 NC_002953:c2129037-2128867 [Staphylococcus aureus subsp. aureus MSSA476]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1