Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 30667..31189 Replicon plasmid pNDM33-3
Accession NZ_CP076650
Organism Escherichia coli strain GD33

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag KP013_RS25285 Protein ID WP_000220561.1
Coordinates 30908..31189 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag KP013_RS25280 Protein ID WP_000121743.1
Coordinates 30667..30918 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP013_RS25260 (KP013_25260) 26790..27143 - 354 WP_024129958.1 DNA distortion polypeptide 3 -
KP013_RS25265 (KP013_25265) 27280..27726 - 447 WP_001074384.1 hypothetical protein -
KP013_RS25270 (KP013_25270) 27730..28572 - 843 WP_000435064.1 replication initiation protein -
KP013_RS25275 (KP013_25275) 28593..29432 - 840 WP_024129959.1 replication initiation protein -
KP013_RS25280 (KP013_25280) 30667..30918 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
KP013_RS25285 (KP013_25285) 30908..31189 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KP013_RS25290 (KP013_25290) 31428..31520 - 93 Protein_36 IS6 family transposase -
KP013_RS25295 (KP013_25295) 31686..32885 - 1200 WP_000948429.1 IS91 family transposase -
KP013_RS25300 (KP013_25300) 32895..33083 - 189 WP_000957857.1 hypothetical protein -
KP013_RS25305 (KP013_25305) 33203..34633 + 1431 Protein_39 IS4-like element IS50R family transposase -
KP013_RS25310 (KP013_25310) 34662..35456 + 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
KP013_RS25315 (KP013_25315) 35477..35713 + 237 Protein_41 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / blaTEM-1B / rmtB / sul1 / qacE / aadA2 / dfrA12 / aph(3')-IIa / oqxB / oqxA - 1..90896 90896


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T206114 WP_000220561.1 NZ_CP076650:30908-31189 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T206114 NZ_CP102141:2098830-2099675 [Streptococcus suis]
ATGAATAAAGCTAAGCTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGATTAACCTTTAATTCCGTGATGACCTA
TTACTTTCTTGAAGTGATTTTGAAAAAATTAAGTCAGAGTACCTATTCAAATCACTATATATTCAAAGGAGGATTCCTAC
TGTCAAATGTTATCGGAGTTGAATCTCGTAGCACGGTTGATATTGATTTTTTGTTCCATCAAATAACACTCTCAGAAGAT
ACTGTGAAGCAGCAACTCAAGGAAATTTTAGCAGATTCCGAAGAAGGTATATCTTTTGTGATTCAATCAATCACAGCTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTTGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATTACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAAAAACTTCAAACCATCTATTCACGTAACTTCTTGAACAGTAGAAG
CAAAGATTTTTACGATGTTTATATTCTTTCAAAACTGAAGAAAGAAGACATTGACTTCATTCAGTTGAGAAATGCCTGTC
AGAGAACATTTTCATATCGCGAAACAGAACTCGATTTTGTAAAGATTATTGAACTACTCGAGAGGTTCAAATCTGACCCT
ATTCAGAATAAACAATGGCAAAATTATTCAAAAAAATATAGCTATACAAAAGGGATTTCACTTGCAGATGTTCTTGATGA
GATGATTCGTCTCATAACAGTTCTCGTTTCTATAAATTCTGATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT206114 WP_000121743.1 NZ_CP076650:30667-30918 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT206114 NZ_CP102141:2098243-2098833 [Streptococcus suis]
ATGTCAAAAAAAGAGATTCTACTTGAATTTATAGAAAACCACAATGGCATTATCACCTATAAAGATTGTAAAGCTCTAAA
GATTCCAACAATATATTTGACAAGACTAGAAAAAGAAGGGATTCTATACCGCGTCGAAAAAGGAATTTTCCTAACCCAGA
ATGGAGACTACGACGAATACTACTTCTTTCAGTACCGTTATCCCAAGGCTATTTTCTCTTATATCTCAGCGCTTTACCTG
CAACAATTTACAGATGAAATTCCTCAATATTTTGACGTAACCATTCCTAGAGGCTACCGTTTTAATACTCCTCCAGCAAA
TCTGAACATTCATTCCGTTTCTAAGGAATATAGCGAACTAGGAATTACACTTGTAAAAACCCCTATGGGAAACGAGGTAA
GAGTGTATGATTTAGAACGTATTATCTGTGATTTTGTGATTCATCGAGAAAAAATAGATACTGAGCTTTTTGTCAAAACA
CTTCAACATTACGGAAACTATTCTAAAAAAAATCTTACAAAACTCTATGAATATGCGACAAAAATGAACACCTTGGACAA
GGTCAAACAAACTCTGGAGGTCCTAGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References