Detailed information of TA system TA206112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 5800..7423
• Replicon: plasmid pNDM33-1
• Accession: NZ_CP076648
• Organism: Escherichia coli strain GD33

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KP013_RS23325
• Protein ID: WP_181726440.1
• Coordinates: 6098..7423 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: KP013_RS23320
• Protein ID: WP_024136327.1
• Coordinates: 5800..6093 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KP013_RS25805	1130..1237	+	108	WP_250950310.1	TerD family protein	-
KP013_RS23295 (KP013_23295)	1672..2913	+	1242	WP_001053338.1	VWA domain-containing protein	-
KP013_RS23300 (KP013_23300)	3476..3757	-	282	WP_000077926.1	hypothetical protein	-
KP013_RS23305 (KP013_23305)	3807..3998	-	192	WP_000398480.1	hypothetical protein	-
KP013_RS23310 (KP013_23310)	4090..4431	-	342	WP_001151575.1	hypothetical protein	-
KP013_RS23315 (KP013_23315)	4804..5196	+	393	WP_000341066.1	hypothetical protein	-
KP013_RS23320 (KP013_23320)	5800..6093	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
KP013_RS23325 (KP013_23325)	6098..7423	+	1326	WP_181726440.1	type II toxin-antitoxin system HipA family toxin	Toxin
KP013_RS23330 (KP013_23330)	7484..7690	+	207	WP_000134171.1	hypothetical protein	-
KP013_RS23335 (KP013_23335)	7792..8202	+	411	WP_000985911.1	hypothetical protein	-
KP013_RS25810	8215..8460	+	246	Protein_12	HNH endonuclease	-
KP013_RS23340 (KP013_23340)	8513..9217	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
KP013_RS23345 (KP013_23345)	9283..9693	+	411	Protein_14	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / qnrS1 / tet(A) / blaTEM-1B / lnu(F) / blaNDM-5 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aph(4)-Ia / aac(3)-IVa / sul3 / aadA2	-	1..266777	266777

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (155-404)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50265.24 Da
• Isoelectric Point: 6.1927

>T206112 WP_181726440.1 NZ_CP076648:6098-7423 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGLGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T206112 NZ_CP102141:c2046555-2046298 [Streptococcus suis]
ATGGGAATCCATTTTACAGAGGATGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAGATTATCAAGGATATGCAACGACACCCGTTTGAAGGTATTGCCAAGCCAGAACCCTTGAAGTATGATTATCAAG
GTGCCTGGTCGAGACGGATTGATGCGGAGAATCGGTTGATTTATATGATGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT206112 WP_024136327.1 NZ_CP076648:5800-6093 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT206112 NZ_CP102141:c2046817-2046557 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAACCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAGCCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTTG
CTGTTGCTCGGAACGCCTACCTATCTCAGAAAGTCTTGCGTGGTATGGCTCAAGTCAAAGCGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1