Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4219005..4219502 Replicon chromosome
Accession NZ_CP076646
Organism Escherichia coli strain GD33

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KP013_RS20485 Protein ID WP_001173343.1
Coordinates 4219005..4219178 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KP013_RS20490 Protein ID WP_000398619.1
Coordinates 4219206..4219502 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP013_RS20475 (4215675) 4215675..4217132 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KP013_RS20480 (4217369) 4217369..4218886 + 1518 WP_001295074.1 lysine--tRNA ligase -
KP013_RS20485 (4219005) 4219005..4219178 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KP013_RS20490 (4219206) 4219206..4219502 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KP013_RS20495 (4219729) 4219729..4220001 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
KP013_RS20500 (4220013) 4220013..4220243 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KP013_RS20505 (4220424) 4220424..4222055 + 1632 WP_001216477.1 sensor histidine kinase -
KP013_RS20510 (4222052) 4222052..4222771 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T206107 WP_001173343.1 NZ_CP076646:c4219178-4219005 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T206107 NZ_CP102140:c1421113-1420931 [Streptococcus suis]
ATGCCTATGACACAAAAAGAAATGGTTAAGCTACTTACCGCCCATGGTTGGACAAAAACTAAAGGTGGTAAGGGGTCTCA
CGTTAAACTTGAAAAAGTAGGCGAGCGACCTATAACCATTCCCCACGGTGAAATTAACAAATACACCGAAAGGGGTATCA
AAAAGCAAGCAGGGCTGTTATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT206107 WP_000398619.1 NZ_CP076646:c4219502-4219206 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT206107 NZ_CP102140:c1420891-1420439 [Streptococcus suis]
ATGCTTGTCACTTATCCAGCTTTATTTTATTATGACGATACAGACGGAGCTACCGCCCCTTATTTTGTCACTTTCCCAGA
CTTTGAACATTCAGCAACTCAGGGCGAAGATATGGCAGACGCTATGGCAATGGCTAGTGATTGGTTAGGTATTCATCTAG
CTGATTATATTGAGAATGGGCGTGATATTCCCACTCCTACCCCTATTAACGCTTTATCATTGGCTGATAATAACCCTTTC
CGTGATGATAAGGACATAGAGCTAGTTTATGACCCTAGCAAGTCTTTTGTATCTATGGTTATGGTTGACGTTGCTGAGTA
TCTAGGCAGTCAAGAGCCTGTAAAGAAAACACTAACTATTCCACGTTGGGCGGATACTTTAGGGCGTGAGTTAGGGCTAA
ATTTCTCACAGACCTTAACAGACGCTATTGCAGATAAGAAAATTCATGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References