Detailed information of TA system TA206106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4042293..4042869
• Replicon: chromosome
• Accession: NZ_CP076646
• Organism: Escherichia coli strain GD33

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: KP013_RS19605
• Protein ID: WP_071447831.1
• Coordinates: 4042582..4042869 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A829G8Z0
• Locus tag: KP013_RS19600
• Protein ID: WP_000063156.1
• Coordinates: 4042293..4042595 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KP013_RS19585 (4038889)	4038889..4041039	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
KP013_RS19590 (4041089)	4041089..4041292	+	204	WP_000467859.1	YbdD/YjiX family protein	-
KP013_RS19595 (4041303)	4041303..4042259	+	957	WP_001297640.1	GTPase	-
KP013_RS19600 (4042293)	4042293..4042595	-	303	WP_000063156.1	BrnA antitoxin family protein	Antitoxin
KP013_RS19605 (4042582)	4042582..4042869	-	288	WP_071447831.1	BrnT family toxin	Toxin
KP013_RS19610 (4043068)	4043068..4043765	-	698	Protein_3844	IS1 family transposase	-
KP013_RS19615 (4043822)	4043822..4043917	+	96	Protein_3845	dienelactone hydrolase family protein	-
KP013_RS19620 (4044063)	4044063..4045295	+	1233	WP_024186283.1	multidrug efflux MFS transporter MdtM	-
KP013_RS19625 (4045336)	4045336..4046616	+	1281	WP_001037400.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11344.85 Da
• Isoelectric Point: 6.8818

>T206106 WP_071447831.1 NZ_CP076646:c4042869-4042582 [Escherichia coli]
MPMEFEWDEYKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T206106 NZ_CP102140:c1418788-1418528 [Streptococcus suis]
ATGTATCATTTAGAGTATTCTAAAAAGGCTCAAAAGCAAATTAAGAAATTAGATAGGCAAATTCAGAGGTTGTTGTTTGC
TTGGATTGATAAACACTTAGAAGGGACTGATAATCCACGCGCCAATGGTAAAGGTTTGACAGGCAATCATGCTAACGAGT
GGCGTTATCGTATCGGAGATTACCGACTGATTTGCGATATTCAAGATGATAAGTTGGTTATTTTAGCACTTGAGTTTGGA
CATCGTAAAGATGTCTATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11521.99 Da
• Isoelectric Point: 9.4646

>AT206106 WP_000063156.1 NZ_CP076646:c4042595-4042293 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT206106 NZ_CP102140:c1419011-1418790 [Streptococcus suis]
ATGAGTACAGTGACAATCAGATTAAATCAAGAAGAAGAAGTATTCTTTAAAAGCTATGCACAATTAACAGGTCAAAGTCT
TTCTAGCCTTTTTAAGAAAGCTTTGGAGCGTGATATTGAAGATGAGTATGATTTAAAAATCTATCATCAAGCTTATGATG
AGTACAAAGCAGATCCAGAAACAATTAGTCATGCTGATTTTAAGAAAGAGCTAGGATTATAG

Structures

Antitoxin

• AlphaFold DB: A0A829G8Z0