Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4554601..4555098 Replicon chromosome
Accession NZ_CP076645
Organism Escherichia coli strain MSI001

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KP725_RS22260 Protein ID WP_001173343.1
Coordinates 4554601..4554774 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KP725_RS22265 Protein ID WP_000398619.1
Coordinates 4554802..4555098 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP725_RS22250 (4551271) 4551271..4552728 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
KP725_RS22255 (4552965) 4552965..4554482 + 1518 WP_001295074.1 lysine--tRNA ligase -
KP725_RS22260 (4554601) 4554601..4554774 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KP725_RS22265 (4554802) 4554802..4555098 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KP725_RS22270 (4555325) 4555325..4555597 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
KP725_RS22275 (4555609) 4555609..4555839 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KP725_RS22280 (4556020) 4556020..4557651 + 1632 WP_021571028.1 sensor histidine kinase -
KP725_RS22285 (4557648) 4557648..4558367 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4510949..4555098 44149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T206086 WP_001173343.1 NZ_CP076645:c4554774-4554601 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T206086 NZ_CP102135:c2016043-2015786 [Streptococcus suis]
ATGGGAATCCATTTTACAGAGGATGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACACTCAAGCGAAT
CAATAAGATTATCAAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GCGCTTGGTCAAGAAGGATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCAGACCAGTTGTATATCTTGTCACTG
AAGGATCATTATGGGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT206086 WP_000398619.1 NZ_CP076645:c4555098-4554802 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT206086 NZ_CP102135:c2016305-2016045 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAACGACGAGTTTGAGCCTTT
AGTTGTGGTCAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTTTTGCGTGGTATGGCTCAAGTCAAAGCGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References