Detailed information of TA system TA020608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1929410..1930186
• Replicon: chromosome
• Accession: NC_002953
• Organism: Staphylococcus aureus subsp. aureus MSSA476

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: SAS_RS09495
• Protein ID: WP_000031108.1
• Coordinates: 1929410..1929562 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: SAS_RS09500
• Protein ID: WP_001251224.1
• Coordinates: 1929587..1930186 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAS_RS09475	1925546..1926367	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
SAS_RS09480	1926828..1928213	-	1386	WP_000116229.1	class II fumarate hydratase	-
SAS_RS09485	1928409..1928804	-	396	WP_000901023.1	hypothetical protein	-
SAS_RS09495	1929410..1929562	-	153	WP_000031108.1	hypothetical protein	Toxin
SAS_RS09500	1929587..1930186	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
SAS_RS09505	1930345..1930815	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
SAS_RS09510	1930820..1931947	-	1128	WP_000379987.1	tRNA epoxyqueuosine(34) reductase QueG	-
SAS_RS09515	1932098..1932820	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
SAS_RS09520	1932813..1934270	-	1458	WP_000649907.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T20608 WP_000031108.1 NC_002953:c1929562-1929410 [Staphylococcus aureus subsp. aureus MSSA476]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T20608 NC_002953:c1929562-1929410 [Staphylococcus aureus subsp. aureus MSSA476]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT20608 WP_001251224.1 NC_002953:c1930186-1929587 [Staphylococcus aureus subsp. aureus MSSA476]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT20608 NC_002953:c1930186-1929587 [Staphylococcus aureus subsp. aureus MSSA476]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4