Detailed information of TA system TA206050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4712180..4712759
• Replicon: chromosome
• Accession: NZ_CP076605
• Organism: Raoultella ornithinolytica strain XF201

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A808YBH6
• Locus tag: KP007_RS22215
• Protein ID: WP_032716505.1
• Coordinates: 4712472..4712759 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: KP007_RS22210
• Protein ID: WP_004857518.1
• Coordinates: 4712180..4712485 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KP007_RS22185 (KP007_22185)	4707387..4708451	-	1065	WP_004857527.1	DUF2955 domain-containing protein	-
KP007_RS22190 (KP007_22190)	4708441..4709508	-	1068	WP_004857525.1	HlyD family secretion protein	-
KP007_RS22195 (KP007_22195)	4709515..4709979	-	465	WP_004857523.1	MarR family transcriptional regulator	-
KP007_RS22200 (KP007_22200)	4710270..4710434	-	165	WP_004857521.1	DUF1127 domain-containing protein	-
KP007_RS22205 (KP007_22205)	4710612..4712024	+	1413	WP_015585221.1	PLP-dependent aminotransferase family protein	-
KP007_RS22210 (KP007_22210)	4712180..4712485	-	306	WP_004857518.1	BrnA antitoxin family protein	Antitoxin
KP007_RS22215 (KP007_22215)	4712472..4712759	-	288	WP_032716505.1	BrnT family toxin	Toxin
KP007_RS22220 (KP007_22220)	4712876..4713295	-	420	WP_004857515.1	FosA family fosfomycin resistance glutathione transferase	-
KP007_RS22225 (KP007_22225)	4713289..4714197	-	909	WP_004857513.1	LysR family transcriptional regulator	-
KP007_RS22230 (KP007_22230)	4714285..4715067	+	783	WP_104896592.1	NAD(P)H-dependent oxidoreductase	-
KP007_RS22235 (KP007_22235)	4715215..4715793	+	579	WP_113991322.1	TetR/AcrR family transcriptional regulator	-
KP007_RS22240 (KP007_22240)	4715937..4716749	+	813	WP_152308912.1	winged helix-turn-helix domain-containing protein	-
KP007_RS22245 (KP007_22245)	4716746..4717264	+	519	WP_015585229.1	FidL-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (35-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11131.57 Da
• Isoelectric Point: 7.5235

>T206050 WP_032716505.1 NZ_CP076605:c4712759-4712472 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRHDRYENGEYRWQTIGLVHGVIVVLVAHSIRFESGTEVIRIIS
ARKADKKERSRYEHG

Nucleotide

• Length: 288 bp

>T206050 NZ_CP102086:c736260-735691 [Proteus mirabilis]
ATGACAAACTATACTGTTTTGCTTGATGCCTGTGTCATGTATCCAGCCCCTCTTAGAAGTTATTTAATGTATCTTGCTAA
TACTGGTTTATTTAGAGCACGTTGGAGTAATCAGATCCATGATGAATGGATCACTAATTTATTAGTCAACAATCCAACAT
TAACAGCTGAGCGACTAATACGAACCAGAGATTTAATGAATGCACATGTTCCTGATTGTTTAGTAGAAGGCTACGAACCG
CTTATTTGTGGTTTAAAGCTACCTGATCCTAATGATCGTCATGTGCTAGTAGCAGCAATAAAAGGACAGGCTCAAGCTAT
AATTACATTTAACTTAAAAGATTTTCCGCAAAAATACCTTAATTCTTTTGATATTTCAGCTATCCATCCTGATGAATTTT
TGAGTGACATGTTTGAATTAAATCCTAGTAGCTGTATTAAAGCGGCGCAACAACATAGACAAAGCCTAAAGAATCCACCA
ATGACCGTAGAGACCTTTTTAGGCTGTTTACAAAAGCAGAAATTACCCCAGTTTATTTCCCATTTACGGGCATTCCAGAT
ATTATTATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11681.22 Da
• Isoelectric Point: 9.4429

>AT206050 WP_004857518.1 NZ_CP076605:c4712485-4712180 [Raoultella ornithinolytica]
MSMVKHKRDNPTALTAQREDELKALAKKSDDDIDYSDIPSSDDEPWSEAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIREQNKKS

Nucleotide

• Length: 306 bp

>AT206050 NZ_CP102086:c736712-736260 [Proteus mirabilis]
ATGAATACGATAAGTGAGAGACTTAGTGCTGAAGATATTGCATTAGCAAAATTAAGTAGCCAAGAGCTATCCGCTGTTAT
GGATATTAATGGCAAAACGCAGAAGCTATCCATTATAGATAAGACGGGTAATAATCATATTGTACAAATACCAAATAGTG
CATTACGCCTAATGATTGAAATTCTTACTCAACTAGGGCAAGGGAATACTGTTAGTATCACACCAATCCATGCTGAGCTA
ACGACACAGGAAGCGGCTGATATACTCAATATGTCCCGTCCTACATTTGTAAAATTATTAGATACACGAGCGATACCCTA
CTCTAGAAAAGGAAATCGTAGGAAAGTTGCTTATGCTGATATTGTTGCTTATAAAAATGAATTGGAGCAAAAACGACTTG
ATGCCCTTGCTGAATTATCGCAATTAGATCAAGAAATGGATATGGGGTATTAA

Structures

Toxin

• AlphaFold DB: A0A808YBH6