Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2863664..2864330 Replicon chromosome
Accession NZ_CP076594
Organism Erwinia amylovora isolate CP20086202

Toxin (Protein)


Gene name cptA Uniprot ID A0A830ZTB8
Locus tag KQH36_RS13035 Protein ID WP_004161808.1
Coordinates 2863664..2864083 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D4HWC2
Locus tag KQH36_RS13040 Protein ID WP_004155593.1
Coordinates 2864064..2864330 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQH36_RS13020 (KQH36_13020) 2859656..2861335 + 1680 WP_004155599.1 type III effector -
KQH36_RS13025 (KQH36_13025) 2861845..2862798 + 954 WP_004155597.1 DMT family transporter -
KQH36_RS13030 (KQH36_13030) 2863028..2863546 + 519 WP_004155596.1 flavodoxin FldB -
KQH36_RS13035 (KQH36_13035) 2863664..2864083 - 420 WP_004161808.1 protein YgfX Toxin
KQH36_RS13040 (KQH36_13040) 2864064..2864330 - 267 WP_004155593.1 FAD assembly factor SdhE Antitoxin
KQH36_RS13045 (KQH36_13045) 2864556..2865542 + 987 WP_004155590.1 tRNA-modifying protein YgfZ -
KQH36_RS13050 (KQH36_13050) 2865543..2866202 - 660 WP_004155588.1 hemolysin III family protein -
KQH36_RS13055 (KQH36_13055) 2866415..2867023 + 609 WP_004155587.1 HD domain-containing protein -
KQH36_RS13060 (KQH36_13060) 2867128..2867857 - 730 Protein_2518 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(8-79)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 16366.46 Da        Isoelectric Point: 11.9444

>T206009 WP_004161808.1 NZ_CP076594:c2864083-2863664 [Erwinia amylovora]
VVLWQCELRPSRLAQRFSLLLHGAVMLALLLPTWPASSGLVRMLLLVLVLLECIRSRRRIGRRQGDIALLEGHELRWRQR
EWCIISRPWLTGQAILLLLQDTHGQRERLWLFADGMEKRNWRQLRLQLLNSKVQGNGWC

Download         Length: 420 bp

>T206009 NZ_CP102065:467437-467553 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGTTGGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10498.07 Da        Isoelectric Point: 6.4605

>AT206009 WP_004155593.1 NZ_CP076594:c2864330-2864064 [Erwinia amylovora]
MDITNKSRIHWACRRGMRELDISIMPFFQYEFDSLNDSDKRLFVRLLESDDPDLFNWLMNHGEPADVELKRMVALIQQRN
KQRGPVAM

Download         Length: 267 bp

>AT206009 NZ_CP102065:c467669-467464 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A830ZTB8

Antitoxin

Source ID Structure
AlphaFold DB A0A831A3F0

References