Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2581355..2582021 Replicon chromosome
Accession NZ_CP076592
Organism Erwinia amylovora isolate CP20140001

Toxin (Protein)


Gene name cptA Uniprot ID A0A830ZTB8
Locus tag KQH33_RS11540 Protein ID WP_004161808.1
Coordinates 2581602..2582021 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D4HWC2
Locus tag KQH33_RS11535 Protein ID WP_004155593.1
Coordinates 2581355..2581621 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQH33_RS11515 (KQH33_11540) 2577828..2578557 + 730 Protein_2229 SDR family oxidoreductase -
KQH33_RS11520 (KQH33_11545) 2578662..2579270 - 609 WP_004155587.1 HD domain-containing protein -
KQH33_RS11525 (KQH33_11550) 2579483..2580142 + 660 WP_004155588.1 hemolysin III family protein -
KQH33_RS11530 (KQH33_11555) 2580143..2581129 - 987 WP_004155590.1 tRNA-modifying protein YgfZ -
KQH33_RS11535 (KQH33_11560) 2581355..2581621 + 267 WP_004155593.1 FAD assembly factor SdhE Antitoxin
KQH33_RS11540 (KQH33_11565) 2581602..2582021 + 420 WP_004161808.1 protein YgfX Toxin
KQH33_RS11545 (KQH33_11570) 2582139..2582657 - 519 WP_004155596.1 flavodoxin FldB -
KQH33_RS11550 (KQH33_11575) 2582887..2583840 - 954 WP_004155597.1 DMT family transporter -
KQH33_RS11555 (KQH33_11580) 2584350..2586029 - 1680 WP_004155599.1 type III effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(8-79)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 16366.46 Da        Isoelectric Point: 11.9444

>T205998 WP_004161808.1 NZ_CP076592:2581602-2582021 [Erwinia amylovora]
VVLWQCELRPSRLAQRFSLLLHGAVMLALLLPTWPASSGLVRMLLLVLVLLECIRSRRRIGRRQGDIALLEGHELRWRQR
EWCIISRPWLTGQAILLLLQDTHGQRERLWLFADGMEKRNWRQLRLQLLNSKVQGNGWC

Download         Length: 420 bp

>T205998 NZ_CP102061:3004109-3004420 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10498.07 Da        Isoelectric Point: 6.4605

>AT205998 WP_004155593.1 NZ_CP076592:2581355-2581621 [Erwinia amylovora]
MDITNKSRIHWACRRGMRELDISIMPFFQYEFDSLNDSDKRLFVRLLESDDPDLFNWLMNHGEPADVELKRMVALIQQRN
KQRGPVAM

Download         Length: 267 bp

>AT205998 NZ_CP102061:3004421-3004711 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A830ZTB8

Antitoxin

Source ID Structure
AlphaFold DB A0A831A3F0

References