Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2225802..2226331 Replicon chromosome
Accession NC_002952
Organism Staphylococcus aureus subsp. aureus MRSA252

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag SAR_RS11235 Protein ID WP_000621176.1
Coordinates 2225802..2226164 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag SAR_RS11240 Protein ID WP_000948331.1
Coordinates 2226161..2226331 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAR_RS11210 2222781..2223551 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
SAR_RS11215 2223526..2224005 - 480 WP_001190825.1 anti-sigma B factor RsbW -
SAR_RS11220 2224007..2224333 - 327 WP_001052491.1 anti-sigma factor antagonist -
SAR_RS11225 2224452..2225453 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
SAR_RS11235 2225802..2226164 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
SAR_RS11240 2226161..2226331 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
SAR_RS11245 2226416..2227564 - 1149 WP_001281140.1 alanine racemase -
SAR_RS11250 2227630..2227989 - 360 WP_000581197.1 holo-ACP synthase -
SAR_RS11255 2227993..2228484 - 492 WP_001286801.1 PH domain-containing protein -
SAR_RS11260 2228477..2230054 - 1578 WP_001294646.1 PH domain-containing protein -
SAR_RS11265 2230047..2230526 - 480 WP_001287082.1 hypothetical protein -
SAR_RS11270 2230735..2231295 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T20599 WP_000621176.1 NC_002952:c2226164-2225802 [Staphylococcus aureus subsp. aureus MRSA252]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T20599 NC_002952:c2226164-2225802 [Staphylococcus aureus subsp. aureus MRSA252]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT20599 WP_000948331.1 NC_002952:c2226331-2226161 [Staphylococcus aureus subsp. aureus MRSA252]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT20599 NC_002952:c2226331-2226161 [Staphylococcus aureus subsp. aureus MRSA252]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References