Detailed information of TA system TA205965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1206560..1207187
• Replicon: chromosome
• Accession: NZ_CP076589
• Organism: Erwinia amylovora isolate CP201324

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: KQH38_RS05460
• Protein ID: WP_004156337.1
• Coordinates: 1206560..1206778 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: KQH38_RS05465
• Protein ID: WP_013035913.1
• Coordinates: 1206798..1207187 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQH38_RS05430 (KQH38_05440)	1202546..1202884	+	339	WP_004156319.1	P-II family nitrogen regulator	-
KQH38_RS05435 (KQH38_05445)	1202902..1204203	+	1302	WP_004172214.1	ammonium transporter AmtB	-
KQH38_RS05440 (KQH38_05450)	1204278..1205138	-	861	WP_004156324.1	acyl-CoA thioesterase II	-
KQH38_RS05445 (KQH38_05455)	1205363..1205920	+	558	WP_004156333.1	YbaY family lipoprotein	-
KQH38_RS05450 (KQH38_05460)	1205943..1206260	-	318	WP_004156334.1	MGMT family protein	-
KQH38_RS05460 (KQH38_05470)	1206560..1206778	-	219	WP_004156337.1	HHA domain-containing protein	Toxin
KQH38_RS05465 (KQH38_05475)	1206798..1207187	-	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
KQH38_RS05470 (KQH38_05480)	1207334..1207687	-	354	WP_004156342.1	hypothetical protein	-
KQH38_RS05475 (KQH38_05485)	1208459..1208605	-	147	WP_004156349.1	type B 50S ribosomal protein L36	-
KQH38_RS05480 (KQH38_05490)	1208593..1208874	-	282	WP_004156350.1	type B 50S ribosomal protein L31	-
KQH38_RS05485 (KQH38_05495)	1209022..1212168	-	3147	WP_004161886.1	multidrug efflux RND transporter permease subunit AcrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T205965 WP_004156337.1 NZ_CP076589:c1206778-1206560 [Erwinia amylovora]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T205965 NZ_CP101940:3996491-3996889 [Salmonella enterica subsp. enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT205965 WP_013035913.1 NZ_CP076589:c1207187-1206798 [Erwinia amylovora]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT205965 NZ_CP101940:3996264-3996491 [Salmonella enterica subsp. enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGATGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Toxin

• AlphaFold DB: A0A831EPQ0