Detailed information of TA system TA205955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 86007..86650
• Replicon: plasmid p19PDR22-KPC4
• Accession: NZ_CP076554
• Organism: Klebsiella pneumoniae strain 19PDR22

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: HF574_RS29990
• Protein ID: WP_001044770.1
• Coordinates: 86007..86423 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: HF574_RS29995
• Protein ID: WP_001261282.1
• Coordinates: 86420..86650 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF574_RS29965 (81281)	81281..82261	+	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
HF574_RS29970 (82243)	82243..82785	+	543	WP_077273850.1	restriction endonuclease	-
HF574_RS29975 (82913)	82913..83470	+	558	WP_042934582.1	hypothetical protein	-
HF574_RS29980 (83890)	83890..84594	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HF574_RS29985 (84647)	84647..85933	-	1287	Protein_115	AAA family ATPase	-
HF574_RS29990 (86007)	86007..86423	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
HF574_RS29995 (86420)	86420..86650	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HF574_RS30000 (86607)	86607..87068	+	462	WP_014343465.1	hypothetical protein	-
HF574_RS30005 (87229)	87229..88173	+	945	WP_011977810.1	hypothetical protein	-
HF574_RS30010 (88210)	88210..88602	+	393	WP_011977811.1	hypothetical protein	-
HF574_RS30015 (88660)	88660..89181	+	522	WP_013214008.1	hypothetical protein	-
HF574_RS30020 (89227)	89227..89430	+	204	WP_011977813.1	hypothetical protein	-
HF574_RS30025 (89460)	89460..90464	+	1005	WP_011977814.1	hypothetical protein	-
HF574_RS30030 (90648)	90648..91427	+	780	WP_013214009.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / rmtB / blaTEM-1B / blaSHV-12	-	1..123686	123686

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T205955 WP_001044770.1 NZ_CP076554:c86423-86007 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T205955 NZ_CP101940:1725010-1725228 [Salmonella enterica subsp. enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT205955 WP_001261282.1 NZ_CP076554:c86650-86420 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT205955 NZ_CP101940:1724608-1724982 [Salmonella enterica subsp. enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3