Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2433705..2434683 Replicon chromosome
Accession NZ_CP076539
Organism Bacillus thuringiensis strain GR007

Toxin (Protein)


Gene name spoIISA Uniprot ID B7IX38
Locus tag KPG64_RS12700 Protein ID WP_000624981.1
Coordinates 2433705..2434442 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID B7IX39
Locus tag KPG64_RS12705 Protein ID WP_000237817.1
Coordinates 2434555..2434683 (+) Length 43 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPG64_RS12680 (KPG64_12680) 2429350..2430132 + 783 WP_016077713.1 class I SAM-dependent methyltransferase -
KPG64_RS12685 (KPG64_12685) 2430290..2431933 - 1644 WP_016077712.1 alpha-keto acid decarboxylase family protein -
KPG64_RS12690 (KPG64_12690) 2432081..2432563 + 483 WP_000191905.1 MarR family winged helix-turn-helix transcriptional regulator -
KPG64_RS12695 (KPG64_12695) 2432729..2433466 + 738 WP_000594159.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
KPG64_RS12700 (KPG64_12700) 2433705..2434442 + 738 WP_000624981.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
KPG64_RS12705 (KPG64_12705) 2434555..2434683 + 129 WP_000237817.1 hypothetical protein Antitoxin
KPG64_RS12710 (KPG64_12710) 2434756..2434932 + 177 WP_000852629.1 stage II sporulation protein SB -
KPG64_RS12715 (KPG64_12715) 2434951..2435340 - 390 WP_000712655.1 YxeA family protein -
KPG64_RS12720 (KPG64_12720) 2435552..2437021 + 1470 WP_000287540.1 beta-Ala-His dipeptidase -
KPG64_RS12725 (KPG64_12725) 2437334..2438143 + 810 WP_016077711.1 papain-like cysteine protease family protein -
KPG64_RS12730 (KPG64_12730) 2438169..2438774 + 606 WP_000517276.1 hypothetical protein -
KPG64_RS12735 (KPG64_12735) 2438915..2439166 - 252 WP_000821942.1 LuxR C-terminal-related transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28335.09 Da        Isoelectric Point: 8.2998

>T205945 WP_000624981.1 NZ_CP076539:2433705-2434442 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T205945 NZ_CP101937:518529-518879 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 43 a.a.        Molecular weight: 4981.37 Da        Isoelectric Point: 3.9150

>AT205945 WP_000237817.1 NZ_CP076539:2434555-2434683 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDALQQVEELEEELKRKS

Download         Length: 129 bp

>AT205945 NZ_CP101937:518243-518524 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6D1SWF1

Antitoxin

Source ID Structure
AlphaFold DB B7IX39

References