Detailed information of TA system TA205944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 238486..239128
• Replicon: chromosome
• Accession: NZ_CP076539
• Organism: Bacillus thuringiensis strain GR007

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: R8I8B8
• Locus tag: KPG64_RS01355
• Protein ID: WP_000635965.1
• Coordinates: 238778..239128 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: R8I8H2
• Locus tag: KPG64_RS01350
• Protein ID: WP_000004570.1
• Coordinates: 238486..238773 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPG64_RS01325 (KPG64_01325)	233803..234765	+	963	WP_016078970.1	UV DNA damage repair endonuclease UvsE	-
KPG64_RS01330 (KPG64_01330)	234758..235330	-	573	WP_016078971.1	rhomboid family intramembrane serine protease	-
KPG64_RS01335 (KPG64_01335)	235424..235783	+	360	WP_000583417.1	holo-ACP synthase	-
KPG64_RS01340 (KPG64_01340)	235940..236890	+	951	WP_062804426.1	outer membrane lipoprotein carrier protein LolA	-
KPG64_RS01345 (KPG64_01345)	237008..238177	+	1170	WP_000390595.1	alanine racemase	-
KPG64_RS01350 (KPG64_01350)	238486..238773	+	288	WP_000004570.1	antitoxin EndoAI	Antitoxin
KPG64_RS01355 (KPG64_01355)	238778..239128	+	351	WP_000635965.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KPG64_RS01360 (KPG64_01360)	239196..241364	+	2169	WP_016078972.1	Tex family protein	-
KPG64_RS01365 (KPG64_01365)	241422..241538	-	117	WP_001143640.1	cortex morphogenetic protein CmpA	-
KPG64_RS01370 (KPG64_01370)	241734..242192	+	459	WP_000343553.1	SprT family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12948.04 Da
• Isoelectric Point: 5.7168

>T205944 WP_000635965.1 NZ_CP076539:238778-239128 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T205944 NZ_CP101936:c1321671-1320925 [Bacillus subtilis subsp. subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTTCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10780.36 Da
• Isoelectric Point: 8.9605

>AT205944 WP_000004570.1 NZ_CP076539:238486-238773 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Nucleotide

• Length: 288 bp

>AT205944 NZ_CP101936:c1320925-1320755 [Bacillus subtilis subsp. subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A366G118

Antitoxin

• AlphaFold DB: A0A366FY90