Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 52385..53028 Replicon plasmid pL3452210II_2
Accession NZ_CP076529
Organism Escherichia coli strain L3452210II

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag KPE21_RS23565 Protein ID WP_001044768.1
Coordinates 52612..53028 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag KPE21_RS23560 Protein ID WP_001261287.1
Coordinates 52385..52615 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE21_RS23550 (KPE21_23550) 47563..48696 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
KPE21_RS23555 (KPE21_23555) 48959..52078 - 3120 WP_023909028.1 hypothetical protein -
KPE21_RS23560 (KPE21_23560) 52385..52615 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KPE21_RS23565 (KPE21_23565) 52612..53028 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
KPE21_RS23570 (KPE21_23570) 53190..55328 - 2139 WP_000350635.1 AAA family ATPase -
KPE21_RS23575 (KPE21_23575) 55793..56788 + 996 WP_000246636.1 hypothetical protein -
KPE21_RS23580 (KPE21_23580) 56831..57724 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId / blaTEM-1B - 1..67321 67321


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T205935 WP_001044768.1 NZ_CP076529:52612-53028 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T205935 NZ_CP101930:525832-526182 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT205935 WP_001261287.1 NZ_CP076529:52385-52615 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT205935 NZ_CP101930:525546-525827 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References