Detailed information of TA system TA205933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 55128..55729
• Replicon: plasmid pL3452210II_1
• Accession: NZ_CP076528
• Organism: Escherichia coli strain L3452210II

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: KPE21_RS23075
• Protein ID: WP_001216045.1
• Coordinates: 55128..55508 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: KPE21_RS23080
• Protein ID: WP_001190712.1
• Coordinates: 55508..55729 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPE21_RS23050 (KPE21_23050)	50569..52053	-	1485	WP_000124150.1	terminase	-
KPE21_RS23055 (KPE21_23055)	52053..53246	-	1194	WP_000219625.1	hypothetical protein	-
KPE21_RS23060 (KPE21_23060)	53332..53784	-	453	WP_001326849.1	late promoter-activating protein	-
KPE21_RS23065 (KPE21_23065)	53873..54916	-	1044	WP_023356283.1	DUF968 domain-containing protein	-
KPE21_RS23070 (KPE21_23070)	54944..55123	-	180	WP_001339207.1	hypothetical protein	-
KPE21_RS23075 (KPE21_23075)	55128..55508	-	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KPE21_RS23080 (KPE21_23080)	55508..55729	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KPE21_RS23085 (KPE21_23085)	55802..56191	-	390	WP_000506730.1	S24 family peptidase	-
KPE21_RS23090 (KPE21_23090)	56366..56605	+	240	WP_223368633.1	hypothetical protein	-
KPE21_RS23095 (KPE21_23095)	56583..56957	+	375	Protein_55	integrase core domain-containing protein	-
KPE21_RS23100 (KPE21_23100)	57528..59384	-	1857	WP_077902027.1	acyltransferase family protein	-
KPE21_RS23105 (KPE21_23105)	59611..60357	+	747	Protein_57	IS1 family transposase	-
KPE21_RS23110 (KPE21_23110)	60456..60635	-	180	Protein_58	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82856	82856
	-	flank	IS/Tn	-	-	56583..56957	374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T205933 WP_001216045.1 NZ_CP076528:c55508-55128 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T205933 NZ_CP101926:c43867-43520 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGGCTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCTATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATAAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT205933 WP_001190712.1 NZ_CP076528:c55729-55508 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT205933 NZ_CP101926:c43520-43221 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAGCAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6