Detailed information of TA system TA205932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4315879..4316504
• Replicon: chromosome
• Accession: NZ_CP076527
• Organism: Escherichia coli strain L3452210II

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KPE21_RS20925
• Protein ID: WP_000911330.1
• Coordinates: 4316106..4316504 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KPE21_RS20920
• Protein ID: WP_000450524.1
• Coordinates: 4315879..4316106 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPE21_RS20895 (4311682)	4311682..4312152	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
KPE21_RS20900 (4312152)	4312152..4312724	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KPE21_RS20905 (4312870)	4312870..4313748	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KPE21_RS20910 (4313765)	4313765..4314799	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
KPE21_RS20915 (4315012)	4315012..4315725	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KPE21_RS20920 (4315879)	4315879..4316106	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KPE21_RS20925 (4316106)	4316106..4316504	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KPE21_RS20930 (4316651)	4316651..4317514	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
KPE21_RS20935 (4317529)	4317529..4319544	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
KPE21_RS20940 (4319618)	4319618..4320316	+	699	WP_000679823.1	esterase	-
KPE21_RS20945 (4320426)	4320426..4320626	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T205932 WP_000911330.1 NZ_CP076527:4316106-4316504 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T205932 NZ_CP101925:c4916045-4915734 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT205932 WP_000450524.1 NZ_CP076527:4315879-4316106 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT205932 NZ_CP101925:c4915733-4915443 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33