Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3880667..3881321 Replicon chromosome
Accession NZ_CP076527
Organism Escherichia coli strain L3452210II

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KPE21_RS18825 Protein ID WP_000244777.1
Coordinates 3880914..3881321 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KPE21_RS18820 Protein ID WP_000354046.1
Coordinates 3880667..3880933 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE21_RS18795 (3875955) 3875955..3876698 + 744 Protein_3676 SDR family oxidoreductase -
KPE21_RS18800 (3876755) 3876755..3878188 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KPE21_RS18805 (3878233) 3878233..3878544 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
KPE21_RS18810 (3878708) 3878708..3879367 + 660 WP_000250274.1 hemolysin III family protein -
KPE21_RS18815 (3879444) 3879444..3880424 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KPE21_RS18820 (3880667) 3880667..3880933 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KPE21_RS18825 (3880914) 3880914..3881321 + 408 WP_000244777.1 protein YgfX Toxin
KPE21_RS18830 (3881361) 3881361..3881882 - 522 WP_001055874.1 flavodoxin FldB -
KPE21_RS18835 (3881994) 3881994..3882890 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KPE21_RS18840 (3882915) 3882915..3883625 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KPE21_RS18845 (3883631) 3883631..3885364 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T205929 WP_000244777.1 NZ_CP076527:3880914-3881321 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205929 NZ_CP101925:c4338078-4337791 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205929 WP_000354046.1 NZ_CP076527:3880667-3880933 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205929 NZ_CP101925:c4337804-4337502 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References