Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3317293..3318093 Replicon chromosome
Accession NZ_CP076527
Organism Escherichia coli strain L3452210II

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag KPE21_RS15990 Protein ID WP_000342449.1
Coordinates 3317566..3318093 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag KPE21_RS15985 Protein ID WP_001277108.1
Coordinates 3317293..3317559 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE21_RS15965 (3312950) 3312950..3313618 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KPE21_RS15970 (3313611) 3313611..3314669 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
KPE21_RS15975 (3314914) 3314914..3315768 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KPE21_RS15980 (3316040) 3316040..3317143 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KPE21_RS15985 (3317293) 3317293..3317559 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KPE21_RS15990 (3317566) 3317566..3318093 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KPE21_RS15995 (3318090) 3318090..3318473 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
KPE21_RS16000 (3318897) 3318897..3320006 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KPE21_RS16005 (3320054) 3320054..3320980 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KPE21_RS16010 (3320977) 3320977..3322254 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
KPE21_RS16015 (3322251) 3322251..3323018 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T205926 WP_000342449.1 NZ_CP076527:3317566-3318093 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T205926 NZ_CP101925:4033489-4033866 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT205926 WP_001277108.1 NZ_CP076527:3317293-3317559 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT205926 NZ_CP101925:4033068-4033442 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References