Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3296002..3296588 Replicon chromosome
Accession NZ_CP076527
Organism Escherichia coli strain L3452210II

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KPE21_RS15865 Protein ID WP_042014162.1
Coordinates 3296002..3296232 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KPE21_RS15870 Protein ID WP_000593555.1
Coordinates 3296229..3296588 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE21_RS15855 (3292039) 3292039..3294774 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
KPE21_RS15860 (3294774) 3294774..3295898 + 1125 WP_001314210.1 ABC transporter permease -
KPE21_RS15865 (3296002) 3296002..3296232 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KPE21_RS15870 (3296229) 3296229..3296588 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KPE21_RS15875 (3296708) 3296708..3297109 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KPE21_RS15880 (3297115) 3297115..3297921 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KPE21_RS15885 (3297918) 3297918..3298682 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KPE21_RS15890 (3298682) 3298682..3299515 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KPE21_RS15895 (3299512) 3299512..3300456 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T205925 WP_042014162.1 NZ_CP076527:3296002-3296232 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T205925 NZ_CP101925:3738630-3738848 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT205925 WP_000593555.1 NZ_CP076527:3296229-3296588 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT205925 NZ_CP101925:3738230-3738604 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References