Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2510830..2511327 Replicon chromosome
Accession NZ_CP076527
Organism Escherichia coli strain L3452210II

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KPE21_RS12275 Protein ID WP_001173343.1
Coordinates 2510830..2511003 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag KPE21_RS12280 Protein ID WP_001425728.1
Coordinates 2511031..2511327 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE21_RS12265 (2507500) 2507500..2508957 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
KPE21_RS12270 (2509194) 2509194..2510711 + 1518 WP_001295074.1 lysine--tRNA ligase -
KPE21_RS12275 (2510830) 2510830..2511003 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KPE21_RS12280 (2511031) 2511031..2511327 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KPE21_RS12285 (2511554) 2511554..2511826 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KPE21_RS12290 (2511838) 2511838..2512068 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
KPE21_RS12295 (2512249) 2512249..2513880 + 1632 WP_001216487.1 sensor histidine kinase -
KPE21_RS12300 (2513877) 2513877..2514596 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T205924 WP_001173343.1 NZ_CP076527:c2511003-2510830 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T205924 NZ_CP101925:c3093232-3092855 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT205924 WP_001425728.1 NZ_CP076527:c2511327-2511031 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT205924 NZ_CP101925:c3093689-3093321 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References