Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1741811..1742429 Replicon chromosome
Accession NZ_CP076527
Organism Escherichia coli strain L3452210II

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KPE21_RS08520 Protein ID WP_001291435.1
Coordinates 1742211..1742429 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KPE21_RS08515 Protein ID WP_000344800.1
Coordinates 1741811..1742185 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE21_RS08505 (1736900) 1736900..1738093 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KPE21_RS08510 (1738116) 1738116..1741265 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KPE21_RS08515 (1741811) 1741811..1742185 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KPE21_RS08520 (1742211) 1742211..1742429 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KPE21_RS08525 (1742601) 1742601..1743152 + 552 WP_000102568.1 maltose O-acetyltransferase -
KPE21_RS08530 (1743268) 1743268..1743738 + 471 WP_000136192.1 YlaC family protein -
KPE21_RS08535 (1743902) 1743902..1745452 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KPE21_RS08540 (1745494) 1745494..1745847 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KPE21_RS08550 (1746226) 1746226..1746537 + 312 WP_000409911.1 MGMT family protein -
KPE21_RS08555 (1746568) 1746568..1747140 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T205921 WP_001291435.1 NZ_CP076527:1742211-1742429 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T205921 NZ_CP101925:2595100-2595378 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT205921 WP_000344800.1 NZ_CP076527:1741811-1742185 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT205921 NZ_CP101925:2595378-2595662 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References