Detailed information of TA system TA205908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 104844..105580
• Replicon: plasmid pK186_1
• Accession: NZ_CP076519
• Organism: Klebsiella pneumoniae strain K186

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: KNW01_RS26635
• Protein ID: WP_004187044.1
• Coordinates: 105098..105580 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: KNW01_RS26630
• Protein ID: WP_003026799.1
• Coordinates: 104844..105110 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNW01_RS26615 (KNW01_26615)	100316..101710	+	1395	WP_032439652.1	cytosine permease	-
KNW01_RS26620 (KNW01_26620)	101722..102930	+	1209	WP_032448288.1	imidazolonepropionase	-
KNW01_RS26625 (KNW01_26625)	102923..103732	+	810	WP_023329017.1	N-formylglutamate deformylase	-
KNW01_RS26630 (KNW01_26630)	104844..105110	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
KNW01_RS26635 (KNW01_26635)	105098..105580	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
KNW01_RS26640 (KNW01_26640)	105791..107137	+	1347	WP_077251107.1	ISNCY family transposase	-
KNW01_RS26645 (KNW01_26645)	107297..108001	+	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
KNW01_RS26650 (KNW01_26650)	108278..109624	+	1347	WP_077254728.1	ISNCY family transposase	-
KNW01_RS26655 (KNW01_26655)	109673..110071	+	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
KNW01_RS26660 (KNW01_26660)	110219..110329	-	111	Protein_112	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mef(B) / aph(3')-Ia	iutA / iucD / iucC / iucB / iucA	1..247566	247566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T205908 WP_004187044.1 NZ_CP076519:105098-105580 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T205908 NZ_CP101922:c3916883-3916596 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT205908 WP_003026799.1 NZ_CP076519:104844-105110 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT205908 NZ_CP101922:c3916609-3916307 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66