Detailed information of TA system TA205907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 57260..57903
• Replicon: plasmid pK186_1
• Accession: NZ_CP076519
• Organism: Klebsiella pneumoniae strain K186

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: KNW01_RS26390
• Protein ID: WP_016236302.1
• Coordinates: 57487..57903 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: KNW01_RS26385
• Protein ID: WP_001261282.1
• Coordinates: 57260..57490 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNW01_RS26350 (KNW01_26350)	52269..53444	+	1176	Protein_51	IS3 family transposase	-
KNW01_RS26355 (KNW01_26355)	53491..53706	+	216	WP_129586912.1	hypothetical protein	-
KNW01_RS26360 (KNW01_26360)	53826..54611	-	786	WP_050485696.1	site-specific integrase	-
KNW01_RS26365 (KNW01_26365)	54657..55178	-	522	WP_032448294.1	hypothetical protein	-
KNW01_RS26370 (KNW01_26370)	55236..55628	-	393	WP_074422814.1	hypothetical protein	-
KNW01_RS26375 (KNW01_26375)	55737..56681	-	945	WP_032448293.1	hypothetical protein	-
KNW01_RS26380 (KNW01_26380)	56881..57303	-	423	WP_050485703.1	hypothetical protein	-
KNW01_RS26385 (KNW01_26385)	57260..57490	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KNW01_RS26390 (KNW01_26390)	57487..57903	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
KNW01_RS26395 (KNW01_26395)	57977..58330	+	354	Protein_60	AAA family ATPase	-
KNW01_RS26400 (KNW01_26400)	58344..59552	-	1209	WP_001352368.1	IS4-like element ISVsa5 family transposase	-
KNW01_RS26405 (KNW01_26405)	59660..60877	+	1218	Protein_62	AAA family ATPase	-
KNW01_RS26410 (KNW01_26410)	60862..61884	+	1023	WP_032439672.1	helicase UvrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mef(B) / aph(3')-Ia	iutA / iucD / iucC / iucB / iucA	1..247566	247566
	-	inside	IScluster/Tn	-	-	52269..59552	7283

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T205907 WP_016236302.1 NZ_CP076519:57487-57903 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T205907 NZ_CP101922:3625691-3626068 [Escherichia coli]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCGTTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCACACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT205907 WP_001261282.1 NZ_CP076519:57260-57490 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT205907 NZ_CP101922:3625281-3625640 [Escherichia coli]
TTGTCGAATAAAATACCGACAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGTCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
GGGATTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3