Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5244734..5245359 Replicon chromosome
Accession NZ_CP076518
Organism Klebsiella pneumoniae strain K186

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KNW01_RS25750 Protein ID WP_002882817.1
Coordinates 5244734..5245117 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KNW01_RS25755 Protein ID WP_004150355.1
Coordinates 5245117..5245359 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNW01_RS25735 (KNW01_25735) 5242100..5243002 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KNW01_RS25740 (KNW01_25740) 5242999..5243634 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KNW01_RS25745 (KNW01_25745) 5243631..5244560 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KNW01_RS25750 (KNW01_25750) 5244734..5245117 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KNW01_RS25755 (KNW01_25755) 5245117..5245359 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KNW01_RS25760 (KNW01_25760) 5245564..5246481 + 918 WP_002882812.1 alpha/beta hydrolase -
KNW01_RS25765 (KNW01_25765) 5246495..5247436 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KNW01_RS25770 (KNW01_25770) 5247481..5247918 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KNW01_RS25775 (KNW01_25775) 5247915..5248775 - 861 WP_002882807.1 virulence factor BrkB family protein -
KNW01_RS25780 (KNW01_25780) 5248769..5249368 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T205906 WP_002882817.1 NZ_CP076518:c5245117-5244734 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T205906 NZ_CP101922:3578543-3578821 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT205906 WP_004150355.1 NZ_CP076518:c5245359-5245117 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT205906 NZ_CP101922:3578280-3578540 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References