Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4663066..4663876 Replicon chromosome
Accession NZ_CP076518
Organism Klebsiella pneumoniae strain K186

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KNW01_RS22975 Protein ID WP_002887280.1
Coordinates 4663066..4663599 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KNW01_RS22980 Protein ID WP_002887278.1
Coordinates 4663610..4663876 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNW01_RS22970 (KNW01_22970) 4661897..4663018 + 1122 WP_002887282.1 cupin domain-containing protein -
KNW01_RS22975 (KNW01_22975) 4663066..4663599 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KNW01_RS22980 (KNW01_22980) 4663610..4663876 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KNW01_RS22985 (KNW01_22985) 4663979..4665412 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
KNW01_RS22990 (KNW01_22990) 4665402..4666085 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KNW01_RS22995 (KNW01_22995) 4666257..4667642 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
KNW01_RS23000 (KNW01_23000) 4667660..4668004 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T205903 WP_002887280.1 NZ_CP076518:c4663599-4663066 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T205903 NZ_CP101922:2594405-2594512 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT205903 WP_002887278.1 NZ_CP076518:c4663876-4663610 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT205903 NZ_CP101922:c2594358-2594292 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References