Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1231908..1232825 Replicon chromosome
Accession NZ_CP076514
Organism Bacillus velezensis strain WB

Toxin (Protein)


Gene name spoIISA Uniprot ID -
Locus tag KNV92_RS06440 Protein ID WP_191073306.1
Coordinates 1232079..1232825 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag KNV92_RS06435 Protein ID WP_003154807.1
Coordinates 1231908..1232078 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNV92_RS06400 (KNV92_06400) 1227141..1228763 + 1623 WP_094247021.1 pyocin knob domain-containing protein -
KNV92_RS06405 (KNV92_06405) 1228776..1229147 + 372 WP_070081681.1 XkdW family protein -
KNV92_RS06410 (KNV92_06410) 1229152..1229349 + 198 WP_071391956.1 XkdX family protein -
KNV92_RS06415 (KNV92_06415) 1229406..1230167 + 762 WP_094247020.1 phage portal protein -
KNV92_RS06420 (KNV92_06420) 1230219..1230482 + 264 WP_003154815.1 hemolysin XhlA family protein -
KNV92_RS06425 (KNV92_06425) 1230496..1230759 + 264 WP_003154813.1 phage holin -
KNV92_RS06430 (KNV92_06430) 1230773..1231651 + 879 WP_045207177.1 N-acetylmuramoyl-L-alanine amidase -
KNV92_RS19265 1231686..1231811 - 126 WP_003154809.1 hypothetical protein -
KNV92_RS06435 (KNV92_06435) 1231908..1232078 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
KNV92_RS06440 (KNV92_06440) 1232079..1232825 - 747 WP_191073306.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
KNV92_RS06445 (KNV92_06445) 1232930..1233928 - 999 WP_003154805.1 inorganic phosphate transporter -
KNV92_RS06450 (KNV92_06450) 1233941..1234558 - 618 WP_003154804.1 DUF47 domain-containing protein -
KNV92_RS06455 (KNV92_06455) 1234844..1236160 - 1317 WP_094247018.1 amino acid permease -
KNV92_RS06460 (KNV92_06460) 1236483..1237433 + 951 WP_077722102.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1198181..1289951 91770


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29047.54 Da        Isoelectric Point: 4.7755

>T205883 WP_191073306.1 NZ_CP076514:c1232825-1232079 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T205883 NZ_CP101921:2716821-2716928 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT205883 WP_003154807.1 NZ_CP076514:c1232078-1231908 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT205883 NZ_CP101921:c2716771-2716708 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTAAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References